Allophycocyanin (APC)-conjugated anti-mouse LY-6AE (Sca-1) (BD Biosciences) was added to a final concentration of 1 µg/106 Lin- cells, then incubated for 30 minutes on ice. Cells were washed with PBS, centrifuged at 300 g for 10 minutes and then passed through a 40-µm filter. Propidium iodide (0.05 mg/mL) was added (1:1,000 dilution), and Lin-/Sca-1+ cells were then separated using a 5 laser Becton Dickinson/Cytopeia Influx High Speed Cell Sorter.
Cytopeia influx high speed cell sorter
Manufactured by BD
The Cytopeia Influx High Speed Cell Sorter is a laboratory instrument designed for the rapid and precise sorting of cells. It utilizes a flow cytometry-based approach to detect and separate specific cell populations based on their physical and fluorescent characteristics.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cytopeia influx high speed cell sorter
1
Isolation of Sca-1+ Hematopoietic Stem Cells
2
Multicolor Flow Cytometry Sorting
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WBM cells (isolated as described above) at 1×107 cells/ml PBS were labelled with either of the following antibodies (0.25 µg/1×106 cells, Invitrogen) on ice for 15 minutes: Anti-Ter119-APC (erythroid cells), CD19-pacific blue (B cells) or Gr-1-APC (granulocytes). Cells were washed with PBS, centrifuged at 300 g for 10 minutes and then passed through a 40-µm filter. Propidium iodide (0.05 mg/mL) was added (1:1,000 dilution), and erythroid cells, B cells and granulocytes were then separated using a 5 laser Becton Dickinson/Cytopeia Influx High Speed Cell Sorter.
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