Antibodies against Iκκβ (ab32135) and p65 (ab7970) and Alexa Fluor 555 Donkey anti-Rb IgG (H + L) antibody (ab150074) were obtained from Abcam (Cambridge, MA, USA). The other antibodies used were as follows: anti-IKB-α (sc-1643) and anti-p-IKB-α (sc-8404) (Santa Cruz Biotechnology, CA, USA), anti-p-p65 (3033S; Cell Signaling Technology, Danvers, MA, USA), and anti-NFKB1 (NF-κB p50) (14220-1-AP; ProteinTech, Rosemont, IL, USA). Anti-GAPDH (H301; TransGen Biotech, Beijing, China) was used as normalized control. Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 (CSB-E04638h), IL-8 (CSB-e04641h), and TNF-α (CSB-EQ023955HU) were obtained from Cusabio Biotech (Wuhan, China). M-MLV Reverse Transcriptase (M1701) and GoTaq qPCR Master Mix (A6001) were obtained from Promega (Madison, WI, USA).
Gotaq qpcr master mix a6001
Manufactured by Promega
Sourced in United States
GoTaq qPCR Master Mix (A6001) is a ready-to-use solution for quantitative PCR (qPCR) reactions. It contains GoTaq DNA Polymerase, PCR buffer, dNTPs, and a fluorescent dye for real-time detection of amplification.
Automatically generated - may contain errors
Lab products found in correlation
Ab7970, by Abcam (1 mentions)
Ab32135, by Abcam (1 mentions)
M mlv reverse transcriptase m1701, by Promega (1 mentions)
Anti ikbα sc 1643, by Santa Cruz Biotechnology (1 mentions)
Anti p p65 3033, by Cell Signaling Technology (1 mentions)
Taurocholic acid sodium salt hydrate, by Merck Group (1 mentions)
Lovastatin, by Merck Group (1 mentions)
Simvastatin, by Merck Group (1 mentions)
L α phosphatidylcholine, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide nad, by Merck Group (1 mentions)
Edta disodium salt dihydrate, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
1 4 dithiothreitol dtt, by Merck Group (1 mentions)
2 protocols using gotaq qpcr master mix a6001
1
NF-κB Activation Pathway Evaluation
2
Cholesterol Metabolism Enzyme Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
cholesterol esterase from Pseudomonas sp., 3-HMG-CoA reductase human, 3-HMG-CoA human, 4-nitrophenyl butyrate
(NPB), cholesterol,l -α-phosphatidylcholine, oleic
acid, lovastatin, cholesterol assay kit (MAK043-1KT), β-nicotinamide
adenine dinucleotide (NAD+), simvastatin, 1,4-dithiothreitol
(DTT), taurocholic acid sodium salt hydrate, gallic acid, and EDTA
disodium salt dihydrate were purchased from Sigma-Aldrich (St Louis,
Missouri, USA) and Glentham LS (Corsham, United Kingdom). Oligo primers
(Inqaba Biotech, RSA), LDL uptake Kit (ab236208 Abcam), SV Total RNA
Isolation (Z3100), and Go Taq qPCR Master Mix (A6001) (Promega) (USA,
Madison, Wisconsin, USA) were used.
(NPB), cholesterol,
acid, lovastatin, cholesterol assay kit (MAK043-1KT), β-nicotinamide
adenine dinucleotide (NAD+), simvastatin, 1,4-dithiothreitol
(DTT), taurocholic acid sodium salt hydrate, gallic acid, and EDTA
disodium salt dihydrate were purchased from Sigma-Aldrich (St Louis,
Missouri, USA) and Glentham LS (Corsham, United Kingdom). Oligo primers
(Inqaba Biotech, RSA), LDL uptake Kit (ab236208 Abcam), SV Total RNA
Isolation (Z3100), and Go Taq qPCR Master Mix (A6001) (Promega) (USA,
Madison, Wisconsin, USA) were used.
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