The chemical groups of PHBV nanofiber scaffolds with different fiber patterns were characterized using a Bruker TENSOR27 FTIR machine (Borken, Germany). The scanning range was 4000~500 cm–1, and the scanning resolution was set to 4 cm–1.
Tensor 27 ftir machine
Manufactured by Bruker
Sourced in Germany, United Kingdom
The Tensor 27 FTIR (Fourier Transform Infrared) machine is a high-performance laboratory instrument designed for infrared spectroscopy analysis. It offers a wide spectral range and advanced optical components to provide accurate and reproducible results. The Tensor 27 FTIR is capable of measuring the infrared absorption or transmission spectra of various samples, including solids, liquids, and gases.
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3 protocols using tensor 27 ftir machine
1
PHBV Nanofiber Scaffold Characterization
2
Calcium Pyrophosphate Crystallization Protocol
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A 50mM sodium pyrophosphate stock solution was created by dissolving sodium pyrophosphate in deionized water, neutralized with 52.1μM NaOH, followed by filtration (0.2 μm PES). This stock solution was made fresh immediately prior to treatment. Calcium pyrophosphate precipitant was obtained by diluting sodium pyrophosphate to a final concentration of 1mM or 100μM in alphaMEM (Gibco), at 1mL/cm2, 37°C in a humidified atmosphere of 5% CO2 for 48 hours. Sodium pyrophosphate formed an insoluble calcium salt at 100μM in alpha-MEM, and between 125–200μM in DMEM media. Crystals were isolated by centrifugation at 3,000g for 1 hour, and air-dried for 48 hours. The x-ray diffraction (XRD) spectra of sodium pyrophosphate precipitant was obtained with a Bruker D8 advanced XRD machine. FTIR spectra were obtained on a Bruker Tensor 27 FTIR machine using a Platinum ATR attachment. SEM images were obtained on a Leo 1550 SEM (Zeiss).
3
FTIR Analysis of GO Dispersion
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A drop of GO dispersion was placed into a Tensor 27 FTIR machine
(Bruker, UK) and dried for 5 min at 60 °C until a powder remained.
Spectroscopic analysis was carried out between 750 and 4000 cm–1. Data processing was completed using OriginPro 8.5.1.
software (Origin Lab, USA).
(Bruker, UK) and dried for 5 min at 60 °C until a powder remained.
Spectroscopic analysis was carried out between 750 and 4000 cm–1. Data processing was completed using OriginPro 8.5.1.
software (Origin Lab, USA).
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