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Goat polyclonal anti biotin and goat anti rabbit alexa fluorvr 680 igg

Manufactured by Thermo Fisher Scientific

Goat polyclonal anti-biotin and goat anti-rabbit Alexa Fluor® 680 IgG are secondary antibodies used in various immunoassay and imaging techniques. The anti-biotin antibody recognizes and binds to biotin, while the Alexa Fluor® 680 antibody binds to rabbit IgG. These reagents can be used to detect and visualize target proteins or molecules in a sample.

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2 protocols using goat polyclonal anti biotin and goat anti rabbit alexa fluorvr 680 igg

1

ACD-Based Proteomic Profiling

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Acid citric dextrose (ACD) tubes were from BD Vacutainer. Ficoll-Paque was purchased from GE Healthcare. Azidohomoalanine (AHA), bicinchonic acid assay kit, Click-it protein reaction kit, biotin alkyne probe, dithiothreitol (DTT), Triton-X100m, C18 tips, goat polyclonal anti-biotin and goat anti-rabbit Alexa FluorVR 680 IgG antibodies were from ThermoFisher. RPMI 1640 Met-, P8340 protease inhibitor cocktail, magnetics streptavidin beads, ammonium bicarbonate (ABC), iodoacetamide (IAA), formic acid (FA), mouse monoclonal anti-actin (clone AC-15), Donkey anti-goat IgG, and goat anti-rabbit IgG HRP-conjugated antibodies were bought from MilliporeSigma. Trypsin/lys-C and the enhanced chemiluminescence kit (ECL) were from Promega and PerkinElmer respectively. Rabbit monoclonal anti-ILK (EP1593Y), rabbit monoclonal anti-ANXA2 (ERP13052B) and mouse monoclonal anti-FERMT3 (3D6) were from Abcam. Mouse monoclonal anti-ATP2A3 (PL/IM430) was from Santa Cruz Biotechnology. Recombinant chicken anti-VCL antibody was from Immune Biosolutions. Goat anti-mouse IgG HRP-conjugated was from Jackson IR. Goat anti-mouse IRDyeVR 800CW IgG was from LI-COR Biosciences.
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2

Optimized Workflow for Proteomic Analysis

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Acid citric dextrose (ACD) tubes were from BD Vacutainer. Ficoll-Paque was purchased from GE Healthcare. Azidohomoalanine (AHA), bicinchonic acid assay kit, Click-it protein reaction kit, biotin alkyne probe, dithiothreitol (DTT), Triton-X100m, C18 tips, goat polyclonal anti-biotin and goat anti-rabbit Alexa FluorVR 680 IgG antibodies were from ThermoFisher. RPMI 1640 Met -, P8340 protease inhibitor cocktail, magnetics streptavidin beads, ammonium bicarbonate (ABC), iodoacetamide (IAA), formic acid (FA), mouse monoclonal anti-actin (clone AC-15), Donkey anti-goat IgG, and goat anti-rabbit IgG HRPconjugated antibodies were bought from MilliporeSigma. Trypsin/lys-C and the enhanced chemiluminescence kit (ECL) were from Promega and PerkinElmer respectively. Rabbit monoclonal anti-ILK (EP1593Y), rabbit monoclonal anti-ANXA2 (ERP13052B) and mouse monoclonal anti-FERMT3 (3D6) were from Abcam. Mouse monoclonal anti-ATP2A3 (PL/IM430) was from Santa Cruz Biotechnology.
Recombinant chicken anti-VCL antibody was from Immune Biosolutions. Goat anti-mouse IgG HRPconjugated was from Jackson IR. Goat anti-mouse IRDyeVR 800CW IgG was from LI-COR Biosciences.
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