30 m db 5 capillary column

Gland extract were analyzed by GC-FID and coupled GC-MS. Samples were injected in splitless mode using a Hewlett Packard 6890 GC, interfaced to a Hewlett Packard 5973 mass selective detector (electron impact, 70 eV). For gland extracts and chemical comparison analysis, a 30-m DB-5 capillary column (0.25-mm ID, 0.25-µm film thickness, J&W Scientific, Folsom, CA) was used. The temperature program was 50°C for 1 min, then rising to 280°C at 10°C/min and holding for 5 min at 280°C. The temperature of the inlet was 250°C, and the transfer line temperature was 280°C. The Wiley MS library (Wiley 2005 ) was installed on the data system. For the analysis of the weathered extracted septa, a 30-m Stabilwax capillary column (0.32-mm ID, 0.25-µm film thickness, Restek, Bellefonte, PA) was used. The temperature program was 100°C for 1 min, then rising to 240°C at 5°C/min and holding for 2 min at 240°C. The temperature of the inlet was 255°C, and FID detector temperature was 255°C. Due to extracted septa compounds unrelated to the Type I, Type II, and ISTDs, which interfered with the residue analysis on the DB-5 capillary column, the Stabilwax column was chosen for the residue analysis. All of the GC analyses used He as carrier gas at constant pressure (41.4 KPa).

Volatile collections were analyzed by GC with flame-ionization detection (FID) and coupled GC-MS. Samples were injected in splitless mode using a Hewlett Packard 6890 GC, interfaced to a Hewlett Packard 5973 mass-selective detector (electron impact, 70 eV). For most analyses, a 30-m DB-5 capillary column (0.25 mm ID, 0.25-µm film thickness, J&W Scientific, Folsom, CA) was used. The temperature program was 50°C for 1 min, then rising to 280°C at 10°C per min, and holding for 5 min at 280°C. The temperature of the inlet was 250°C, and the transfer line temperature was 280°C. The Wiley MS library (Wiley 2005 ) was installed on the data system. All the GC analyses used He as carrier gas at constant pressure (41.4 KPa).