Tcrβ clone h57 597

Manufactured by Cytek Biosciences

The TCRβ (clone: H57–597) is a lab equipment product offered by Cytek Biosciences. It is designed to detect and analyze T cell receptor beta chains, which are essential components of the T cell receptor complex. The core function of this product is to provide researchers with a reliable and specific tool for the identification and characterization of T cell populations.

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Lab products found in correlation

Cd45.2 clone 104, by BioLegend (2 mentions) Cd44 clone im7, by BioLegend (2 mentions) Cd62l clone mel 14, by BioLegend (2 mentions) Cd8 clone 53 6, by Cytek Biosciences (2 mentions) Mhcii clone kh74, by BioLegend (2 mentions) Nk1.1 clone pk136, by Thermo Fisher Scientific (2 mentions) Cd4 clone rm4 5, by BioLegend (2 mentions) Gallios and kaluza software, by Beckman Coulter (2 mentions) Cd11b clone m1 70, by BioLegend (2 mentions) F4 80 clone bm8, by BioLegend (2 mentions) Attune nxt cytometer, by Thermo Fisher Scientific (1 mentions) Cd11c clone n418, by Cytek Biosciences (1 mentions) Tcrγ δ clone gl3, by BioLegend (1 mentions) Cd19 clone 1d3, by Cytek Biosciences (1 mentions) Cd8α clone 53 6.7, by Cytek Biosciences (1 mentions)

3 protocols using tcrβ clone h57 597

Isolation and Characterization of Immune Cells from IKKβ Liver

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ELSs were dissected under binocular from IKKβ(EE)^Hep livers and digested for 30 minutes in 500µl digestion buffer (HBSS with 0.2 mg/ml collagenase IV and 0.1 mg/ml DNase1) at 37°C with gentle agitation. The cells were strained through 40µm filter by washing with cold DMEM, centrifuged for 15 minutes, RBCs were lysed for 10 minutes at 25° with erythrocytes lysis buffer, washed again and resuspended in 0.5ml DMEM and kept on ice for a few hours until staining. Viability of isolated immune cells was around 85% as determined by Trypan blue. Cells were resuspended and stained in PBS supplemented with 1% fetal calf serum and 1mM EDTA. Samples were stained and then analyzed by flow cytometry using a Gallios and Kaluza software (Beckman Coulter), or by fluorescence-activated cell sorter (FACS). Antibodies used for flow cytometry and FACS analysis: CD4 (clone RM-4.5, catalog#: 100536, BioLegend), CD8 (clone 53–6.7, catalog#: 65–0081 and 75–008, Tonbo), F4/80 (clone BM8, catalog#: 123127, BioLegend), CD11b (clone M1/70, catalog#: 101224, BioLegend), MHCII (clone KH74, catalog#: 115303, BioLegend), CD45.2 (clone 104, catalog#: 109807, BioLegend), NK1.1 (clone PK136, catalog#: 12–5941-83, eBioscience), TCRβ (clone: H57–597, catalog#: 35–5961, Tonbo), CD44 (clone IM7, catalog#: 103127, BioLegend), CD62L (clone MEL-14, catalog#: 104417, BioLegend), B220 (Catalog#: 553090, BD Pharmingen).

Finkin S., Yuan D., Stein I., Taniguchi K., Weber A., Unger K., Browning J.L., Goossens N., Nakagawa S., Gunasekaran G., Schwartz M.E., Kobayashi M., Kumada H., Berger M., Pappo O., Rajewsky K., Hoshida Y., Karin M., Heikenwalder M., Ben-Neriah Y, & Pikarsky E. (2015). Ectopic lymphoid structures function as microniches for tumor progenitor cells in hepatocellular carcinoma. Nature immunology, 16(12), 1235-1244.

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Isolation and Characterization of Immune Cells from IKKβ(EE)Hep Liver

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ELSs were dissected under binocular from IKKβ(EE)^Hep livers and digested for 30 minutes in 500μl digestion buffer (HBSS with 0.2 mg/ml collagenase IV and 0.1 mg/ml DNase1) at 37°C with gentle agitation. The cells were strained through 40μm filter by washing with cold DMEM, centrifuged for 15 minutes, RBCs were lysed for 10 minutes at 25° with erythrocytes lysis buffer, washed again and resuspended in 0.5ml DMEM and kept on ice for a few hours until staining. Viability of isolated immune cells was around 85% as determined by Trypan blue. Cells were resuspended and stained in PBS supplemented with 1% fetal calf serum and 1mM EDTA. Samples were stained and then analyzed by flow cytometry using a Gallios and Kaluza software (Beckman Coulter), or by fluorescence-activated cell sorter (FACS). Antibodies used for flow cytometry and FACS analysis: CD4 (clone RM-4.5, catalog#: 100536, BioLegend), CD8 (clone 53-6.7, catalog#: 65-0081 and 75-008, Tonbo), F4/80 (clone BM8, catalog#: 123127, BioLegend), CD11b (clone M1/70, catalog#: 101224, BioLegend), MHCII (clone KH74, catalog#: 115303, BioLegend), CD45.2 (clone 104, catalog#: 109807, BioLegend), NK1.1 (clone PK136, catalog#: 12-5941-83, eBioscience), TCRβ (clone: H57-597, catalog#: 35-5961, Tonbo), CD44 (clone IM7, catalog#: 103127, BioLegend), CD62L (clone MEL-14, catalog#: 104417, BioLegend), B220 (Catalog#: 553090, BD Pharmingen).

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Quantification of Hematopoietic Stem Cells

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Single cell suspensions from bone marrow were prepared and erythrocytes were lysed in ACK lysis buffer. Live single cells were analyzed by flow cytometry to quantify the Lin⁻Sca-1⁺c-Kit⁺ (LSK) population. Fixable viability dye was from Tonbo. The lineage cocktail was comprised of APC-conjugated antibodies against CD8α(clone 53-6.7, Tonbo), TCRβ (clone H57-597, Tonbo), TCRγδ (clone Gl3, Biolegend), CD3ε (clone 145-2C11, Tonbo), B220 (clone RA3-682, Tonbo), CD19 (clone 1D3, Tonbo), CD11c (clone N418, Tonbo), Gr-1 (clone RB6-8C5, Tonbo), NK1.1 (clone PK136, Tonbo) and Ter119 (clone Ter119, Tonbo). Sca-1 (clone D7) and c-Kit (clone 2B8) were purchased from Biolegend or eBioscience. Analysis was performed on an Attune NxT cytometer (Life Technologies) and was analyzed using FlowJo (TreeStar).

Shapiro M.J., Lehrke M.J., Chung J.Y., Arocha S.R, & Shapiro V.S. (2019). NKAP must associate with HDAC3 to regulate hematopoietic stem cell maintenance and survival. Journal of immunology (Baltimore, Md. : 1950), 202(8), 2287-2295.

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