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Cell culture medium and supplements

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Cell culture medium and supplements are essential components for the in vitro cultivation and maintenance of cells. They provide the necessary nutrients, growth factors, and other essential elements to support the growth and proliferation of various cell types.

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Cell culture medium (35 citations) Cell culture supplements (9 citations)

2 protocols using cell culture medium and supplements

1

Culturing Human Cardiomyocyte Cell Line AC16

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The human cardiomyocyte cell line (AC16) was purchased from MilliporeSigma (Burlington, MA, United States). AC16 cells were maintained and grown in DMEM/F12 supplemented with 2 mM EmbryoMax L-glutamine, 12.5% EmbryoMax fetal bovine serum (FBS) and 1% of EmbryoMax Penicillin–Streptomycin Solution at 37°C in a humidified atmosphere of 5% carbon dioxide (CO2). Cell culture medium and supplements were purchased from Sigma-Aldrich (St. Louis, MO, United States).
Wei Z., Zhao J., Niebler J., Hao J.J., Merrick B.A, & Xia M. (2020). Quantitative Proteomic Profiling of Mitochondrial Toxicants in a Human Cardiomyocyte Cell Line. Frontiers in Genetics, 11, 719.
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2

Reagent Sourcing for Cell Culture

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Chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Carl Roth (Karlsruhe, Germany). Cell culture medium and supplements were purchased from Sigma-Aldrich or Gibco (Thermo Fisher Scientific, Waltham, MA, USA).
Weng W., Zanetti F., Bovard D., Braun B., Ehnert S., Uynuk-Ool T., Histing T., Hoeng J., Nussler A.K, & Aspera-Werz R.H. (2021). A simple method for decellularizing a cell-derived matrix for bone cell cultivation and differentiation. Journal of Materials Science. Materials in Medicine, 32(9), 124.
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