Efficient navigation

Images were obtained in eight-well NUNC chambers (Thermo Scientific) including 250 μl of GMPV stock solution. For experiments in the presence of ADM-116_F or ADM-3_F, 200 nM of small molecule was incubated in the GMPV solution for 24 h at room temperature. Imaging was carried out at the Yale Department of Molecular, Cellular and Developmental Biology imaging facility on a Zeiss LSM 510 confocal microscope, using a × 63 Plan-Apo/1.4-NA oil-immersion objective with DIC capability (Carl Zeiss). For all experiments, the gain setting for the green channel was kept constant from sample to sample. Image acquisition and processing were achieved using Zeiss Efficient Navigation and Image J software46 (link).

Images were obtained in eight-well NUNC chambers (Thermo Scientific, Rochester, NY, USA) seeded with 20,000–25,000 cells per well. After culturing for 48 h, the medium was replaced with the medium containing constituents according to the experiment performed. For time-dependent localization experiments of IAPP, the medium contained 100 nM IAPP_A594, 13 μM unlabelled peptide and incubated for the specified time points. For experiments in the presence of ADM-116_F and ADM-3_F, additional fluorescein-labelled and -unlabelled small molecules, 200 and 13 μM, respectively, was introduced in the medium. For delayed addition experiment with small molecules, the medium was removed for the second time, replacing with medium containing the small molecule. Images were acquired after 48-h total incubation time. Imaging was carried out at the Yale Department of Molecular, Cellular and Developmental Biology imaging facility on a Zeiss LSM 510 confocal microscope, using a × 63 Plan-Apo/1.4-NA oil-immersion objective with DIC capability (Carl Zeiss, Oberkochen, Germany). For all experiments reporting on the uptake of labelled IAPP, the gain setting for the red channel was kept constant from sample to sample. Image acquisition and processing were achieved using Zeiss Efficient Navigation and Image J software.