Rabbit anti sp a

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit anti-SP-A is an antibody product that recognizes the surfactant protein A (SP-A) antigen. SP-A is a protein found in the lungs and plays a role in the immune function of the respiratory system. This antibody can be used in various research applications to detect and study SP-A.

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Lab products found in correlation

Immobilon p, by Merck Group (1 mentions) Rabbit anti actin, by Cell Signaling Technology (1 mentions) Gradient gel, by Bio-Rad (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Precellys 24 homogenizer, by Bertin Technologies (1 mentions) Ck14 tubes, by Bertin Technologies (1 mentions) Rabbit anti pdgfrβ, by Cell Signaling Technology (1 mentions) Rabbit anti sp c, by Hycult Biotech (1 mentions)

Spelling variants of this product

Anti–SP‐A (2 citations) Rabbit anti-TM (2 citations)

2 protocols using rabbit anti sp a

Western Blot Analysis of Lung Proteins

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For western blots, one snap-frozen lung lobe was lysed in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% deoxycholate, 0.5% SDS, 0.1% NP-40, 0.1% Triton X-100, supplemented with PhosSTOP phosphatase inhibitor and Complete protease inhibitor cocktail (both Roche Diagnostics), using a Precellys24 homogenizer and CK14 tubes (Bertin Technologies SAS, Montigny le Bretonneux, France). Tissue debris were removed from the lysates by centrifugation at 4°C for 30 min at 10,000 rpm 10 μg of total proteins were separated by SDS-PAGE on 4–12% gradient gels (Biorad) and transferred to PVDF membranes (Immobilon-P, Millipore). For the detection of proteins, the following primary antibodies were used: goat anti-SP-C, rabbit anti-SP-A (Santa Cruz Biotechnologies, 1:500), rabbit anti-Actin (Cell Signaling Technologies, 1:2000), rabbit anti-calnexin ([44 (link)], 1:5000).

Niaudet C., Hofmann J.J., Mäe M.A., Jung B., Gaengel K., Vanlandewijck M., Ekvärn E., Salvado M.D., Mehlem A., Al Sayegh S., He L., Lebouvier T., Castro-Freire M., Katayama K., Hultenby K., Moessinger C., Tannenberg P., Cunha S., Pietras K., Laviña B., Hong J., Berg T, & Betsholtz C. (2015). Gpr116 Receptor Regulates Distinctive Functions in Pneumocytes and Vascular Endothelium. PLoS ONE, 10(9), e0137949.

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Quantitative Protein Analysis in Lung Tissue

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Western blotting analysis was performed using the ECL system. The following primary antibodies were used: rabbit anti-PDGFRα, rabbit anti-PDGFRβ (Cell Signalling Technology), rabbit anti-Pad4 (MBL), rabbit anti-SP-C (Hycult Biotech), goat anti-SP-D (R&D Systems, Minneapolis, MN, USA), rabbit anti-COL1A1 (Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-SP-A and mouse anti-tubulin-α (Santa Cruz Biotechnology, Dallas, TX, USA) antibodies. Statistical analysis of the expression levels of each protein was performed using Fiji.

Miura Y., Ohkubo H., Niimi A, & Kanazawa S. (2021). Suppression of epithelial abnormalities by nintedanib in induced-rheumatoid arthritis-associated interstitial lung disease mouse model. ERJ Open Research, 7(4), 00345-2021.

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