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Phycoerythrin annexin 5 apoptosis detection kit with 7 aminoactinomycin d

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The Phycoerythrin Annexin V Apoptosis Detection Kit with 7‐aminoactinomycin D is a laboratory reagent that can be used to detect and analyze apoptosis in cells. It contains Annexin V conjugated to the fluorescent dye phycoerythrin and the nuclear stain 7‐aminoactinomycin D.

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Lab products found in correlation

Facscalibur system, by BD (1 mentions)

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Annexin V (328 citations) 7-aminoactinomycin D (72 citations) Apoptosis detection kit (47 citations) Annexin V Apoptosis Detection Kit (30 citations) Annexin V detection kit (5 citations) Annexin V kit (4 citations)

2 protocols using phycoerythrin annexin 5 apoptosis detection kit with 7 aminoactinomycin d

1

Apoptosis Induction in Cell Lines

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TM3, TM4, and GC‐1 cells were treated with NCL1 for 48 hours. A Guava® easyCyte system (Luminex Corporation) was used as an apoptosis assay. A phycoerythrin Annexin V Apoptosis Detection Kit with 7‐aminoactinomycin D (BioLegend) was used as an apoptosis assay in accordance with the manufacturer's instructions.
Nozaki S., Naiki T., Naiki‐Ito A., Iwatsuki S., Takeda T., Etani T., Nagai T., Iida K., Kato H., Suzuki T., Takahashi S., Umemoto Y, & Yasui T. (2020). Selective lysine‐specific demethylase 1 inhibitor, NCL1, could cause testicular toxicity via the regulation of apoptosis. Andrology, 8(6), 1895-1906.
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2

Luteolin-Induced Apoptosis and Cell Cycle

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Cells were treated with luteolin for 48 hours and collected in 6‐well plates. A FACSCalibur system (BD) was used for both apoptosis and cell‐cycle assays. We used a phycoerythrin Annexin V Apoptosis Detection Kit with 7‐aminoactinomycin D (BioLegend) for apoptosis assays, and a Cell Cycle Phase Detection Kit (Cayman Chemical) to evaluate the cell cycle by propidium iodide according to the manufacturer’s instructions. All experiments were performed in triplicate.
Iida K., Naiki T., Naiki‐Ito A., Suzuki S., Kato H., Nozaki S., Nagai T., Etani T., Nagayasu Y., Ando R., Kawai N., Yasui T, & Takahashi S. (2020). Luteolin suppresses bladder cancer growth via regulation of mechanistic target of rapamycin pathway. Cancer Science, 111(4), 1165-1179.
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