A Nanalysis Benchtop 60‐MHz NMR, a Varian Inova 500‐MHz NMR spectrometer equipped with FTS Systems TC‐84 Kinetics Air Jet Temperature Controller were used to collect 1H‐NMR spectra. A Varian Mercury 300‐MHz NMR spectrometer operating at 75 MHz was used to collect 13C‐NMR spectra. Varian Inova 400‐MHz spectrometer equipped with a 5‐mm broad‐band probe operating at a resonance frequency of 54.24 MHz were used for 17O‐NMR spectroscopy experiments. All pH measurements were performed by using an Orion 8115BNUWP Ross Ultra Semi Micro pH electrode connected to a 702 SM Titrino pH. A Thermo Finnigan LCQ Advantage (Thermofisher, Waltham, MA, USA) with ESI ionization and Surveyor HPLC and a 12T Bruker SolariXR 12 Hybrid FTMS (Bruker Scientific, Billerica, MA, USA) with Imaging MALDI and Nano‐LC were used for analyzing masses of the complexes and the ligands. Iron concentration was determined using a ThermoElectron X‐Series 2 ICP‐MS (Thermofisher, Waltham, MA, USA). Absorbance spectra were recorded on a Beckman‐Coulter DU 800 UV–Vis Spectrophotometer (Beckman Coulter, Pasadena, CA, USA) equipped with a Peltier temperature controller.
Thermo finnigan lcq advantage
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Thermo Finnigan LCQ Advantage is a liquid chromatography-mass spectrometry (LC-MS) system designed for analytical applications. It provides accurate mass measurements and high-sensitivity detection for a wide range of samples.
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Lab products found in correlation
5 mm broadband probe, by Agilent Technologies (1 mentions)
Mercury 300 mhz nmr spectrometer, by Agilent Technologies (1 mentions)
Inova 400 mhz spectrometer, by Agilent Technologies (1 mentions)
Methanol d4, by Eurisotop (1 mentions)
Gemini c18 column, by Phenomenex (1 mentions)
Agilent 1100 series, by Agilent Technologies (1 mentions)
Avance 3 500 mhz spectrometer, by Bruker (1 mentions)
2 protocols using thermo finnigan lcq advantage
1
Multimodal Spectroscopic Analysis of Complexes
2
Comprehensive HPLC-ESI-MS and NMR Analysis
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HPLC-ESI-MS analysis. HPLC-ESI-MS was carried out in switch (positive/negative) ion mode using an Agilent 1100 Series liquid chromatography HPLC system (Agilent, Palo Alto, CA, USA) coupled with a Thermo Finnigan LCQ Advantage ion trap mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) and equipped with an ESI source. The HPLC system included a binary pump, a thermostated autosampler, a column oven, and a diode array detector. A Gemini C18 column (150 mm × 3 mm, 3 µm; Phenomenex, Torrance, CA, USA) was used with an injection volume of 5 L and a flow rate of 0.3 mL/min. All samples were filtered through 0.2 µm sterile membranes before HPLC-MS analysis. The HPLC gradient used water (A) and ACN (B) (both acidified with 0.1% formic acid) as follows: 10%-100% B in 10 minutes and 100%-100% B in 4 minutes. The MS instrument parameters were: nebulization gas, N 2 , 50 a.u. sheath and 10 a.u. auxiliary gas flow; spray voltage, ±4.5 kV; capillary temperature, 275 °C; capillary voltage, -10 V; tube lens offset, -50 V.
NMR Analysis. NMR spectra of daphnetin and daphnoretin were acquired on a Bruker 500 MHz Avance III spectrometer (Bruker Biospin, Rheinstetten, Germany). Methanol-d4 (Euriso-Top, Saint-Aubin, France) was used as a deuterated solvent and its protonated residual signals were used as an internal standard at 3.31 ppm relative to trimethylsilane.
NMR Analysis. NMR spectra of daphnetin and daphnoretin were acquired on a Bruker 500 MHz Avance III spectrometer (Bruker Biospin, Rheinstetten, Germany). Methanol-d4 (Euriso-Top, Saint-Aubin, France) was used as a deuterated solvent and its protonated residual signals were used as an internal standard at 3.31 ppm relative to trimethylsilane.
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