4 6 diamidino 2 phenylindole dapi staining reagents

Manufactured by Thermo Fisher Scientific

4′,6-diamidino-2-phenylindole (DAPI) is a fluorescent staining reagent used for labeling and visualizing nucleic acids, particularly DNA, in various biological samples. It binds strongly to the minor groove of double-stranded DNA, emitting a blue fluorescence when excited by ultraviolet or violet light.

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Lab products found in correlation

Ab20272, by Abcam (1 mentions) Alexa 594 phalloidin, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase labeled secondary antibody, by Santa Cruz Biotechnology (1 mentions) Ab21685, by Merck Group (1 mentions) Alexa 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti myc, by Santa Cruz Biotechnology (1 mentions) Mouse anti sma, by Merck Group (1 mentions) Rabbit anti c myc, by Merck Group (1 mentions) Rabbit anti yap1, by Cell Signaling Technology (1 mentions) Alexa 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Hrp labeled secondary antibody, by Santa Cruz Biotechnology (1 mentions) Ab2302, by Abcam (1 mentions)

2 protocols using 4 6 diamidino 2 phenylindole dapi staining reagents

Immunofluorescence Staining of Synaptopodin

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The following primary antibodies were used: mouse monoclonal anti-SYNPO (G1D4; PROGEN, Heidelberg, Germany), rabbit anti-SYNPO (PA5–56997; Thermo Fisher Scientific), mouse horseradish peroxidase–linked anti-beta actin (ab20272; Abcam, Cambridge, UK). Donkey anti-mouse, anti-rabbit Alexa 488– and Alexa 594–conjugated secondary antibodies; 4′,6-diamidino-2-phenylindole (DAPI) staining reagents; Phalloidin-Alexa 594 were obtained from Invitrogen (Thermo Fisher Scientific). Horseradish peroxidase–labeled secondary antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX).

Klämbt V., Mao Y., Schneider R., Buerger F., Shamseldin H., Onuchic-Whitford A.C., Deutsch K., Kitzler T.M., Nakayama M., Majmundar A.J., Mann N., Hugo H., Widmeier E., Tan W., Rehm H.L., Mane S., Lifton R.P., Alkuraya F.S., Shril S, & Hildebrandt F. (2020). Generation of Monogenic Candidate Genes for Human Nephrotic Syndrome Using 3 Independent Approaches. Kidney International Reports, 6(2), 460-471.

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Comprehensive Antibody Characterization for Cell-Based Studies

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The following primary antibodies were used: rabbit anti-NOS1AP (Novus Biologicals, NBP2-38758 and NBP2-38151), guinea anti-nephrin (Progen, GP-N2), mouse anti-SMA (Sigma-Aldrich, A2547), mouse anti-CD31 (MA3100, Thermo Fisher Scientific), mouse anti-NWASP (LSBio, LS-C133098-100), mouse anti-DIAPH3 (Proteintech, 14342-1-AP), rabbit anti-c-Myc (Sigma-Aldrich, C3956), mouse anti-myc (Santa Cruz Biotechnology, SC-40), mouse horseradish peroxidase (HRP)–linked anti–β-actin (Abcam, ab20272), rabbit anti-YAP1 (Cell Signaling Technology, 4912), anti-Golgin B1 (GOLGB1) (Sigma-Aldrich, HPA011008), anti-GRP78 Binding Immunoglobulin Protein (BiP) (Abcam, ab21685), anti-Nucleoporin 153 (NUP153) (Sigma-Aldrich, HPA027897), and anti–cleaved CASP3 (Abcam, ab2302). Donkey anti-mouse, anti-guinea, and anti-rabbit Alexa 488– and Alexa 594–conjugated secondary antibodies; 4′,6-diamidino-2-phenylindole (DAPI) staining reagents; and phalloidin–Alexa 488 were obtained from Invitrogen (Thermo Fisher Scientific). HRP-labeled secondary antibodies were purchased from Santa Cruz Biotechnology.

Majmundar A.J., Buerger F., Forbes T.A., Klämbt V., Schneider R., Deutsch K., Kitzler T.M., Howden S.E., Scurr M., Tan K.S., Krzeminski M., Widmeier E., Braun D.A., Lai E., Ullah I., Amar A., Kolb A., Eddy K., Chen C.H., Salmanullah D., Dai R., Nakayama M., Ottlewski I., Kolvenbach C.M., Onuchic-Whitford A.C., Mao Y., Mann N., Nabhan M.M., Rosen S., Forman-Kay J.D., Soliman N.A., Heilos A., Kain R., Aufricht C., Mane S., Lifton R.P., Shril S., Little M.H, & Hildebrandt F. (2021). Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice. Science Advances, 7(1), eabe1386.

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