5 × 104 cells in serum-free RPMI 1640 were seeded in the upper chamber (8 μm; Millipore), and RPMI 1640 containing 10% FBS was added into the lower chamber. After culturing for 24 h, the cells migrated through the membrane were fixed with methanol and stained with 0.1% crystal violet. The images were taken by an IX7 inverted microscope (Olympus, Tokyo, Japan). All experiments were conducted in triplicate.
Ix7 inverted microscope
Manufactured by Olympus
Sourced in Japan
The IX7 inverted microscope is a laboratory equipment designed for high-performance imaging and observation. It features a stable inverted design and supports a wide range of contrast methods, including brightfield, phase contrast, and fluorescence microscopy. The IX7 provides reliable and precise performance for various applications in cell biology, tissue culture, and other life science research fields.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ix7 inverted microscope
1
Cell Migration Assay Protocol
2
Cell Migration Assay in Serum-free RPMI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the indicated treatment in serum-free RPMI DMEM, 5 × 104 cells were seeded in the upper chamber (8 mm; Millipore), and RPMI DMEM containing 10% FBS was added to the lower chamber. After culturing for 24 h, the cells that had migrated through the membrane were fixed with methanol and stained with 0.1% crystal violet. Images were taken using an IX7 inverted microscope (Olympus, Tokyo, Japan). All experiments were conducted in triplicate.
3
Cell Migration Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Coming to the migration assays, at 48 h post-transfection, 5 × 104 cells in serum-free media were put into the upper chamber (8 μm pore size; Millipore) of the insert, the lower chamber was added with medium containing 10% FBS. After 24 h-incubation, the cells contained in the upper membrane were removed by cotton wool. The cells that migrated through the membrane were stained with methanol and 0.1% crystal violet, imaged, and counted using an IX7 inverted microscope (Olympus, Tokyo, Japan). Experiments were conducted three times independently.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!