Right hemispheres were processed for RNA and/or protein extraction (see
below). Left hemispheres were immersion fixed in 4% paraformaldehyde (PFA) at
4°C for 24 h, washed in PBS, cryoprotected in 30% sucrose in PBS at
4°C, embedded and frozen in
M1 matrix (Thermo Fisher Scientific) on dry
ice and sectioned coronally at 16 μm thickness on a
cryostat (Leica
Biosystems). Sections were mounted on
Superfrost Plus charged slides (Thermo
Fisher Scientific), allowed to dry overnight and stored at -80°C.
White M.A., Kim E., Duffy A., Adalbert R., Phillips B.U., Peters O.M., Stephenson J., Yang S., Massenzio F., Lin Z., Andrews S., Segonds-Pichon A., Metterville J., Saksida L.M., Mead R., Ribchester R.R., Barhomi Y., Serre T., Coleman M.P., Fallon J., Bussey T.J., Brown RH J.r, & Sreedharan J. (2018). TDP-43 gains function due to perturbed autoregulation in a Tardbp knock-in mouse model of ALS-FTD. Nature neuroscience, 21(4), 552-563.