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Tri carb 2900tr instrument

Manufactured by PerkinElmer
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The Tri-Carb 2900TR is a liquid scintillation counter manufactured by PerkinElmer. It is a laboratory instrument used for the detection and quantification of radioactive samples.

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Lab products found in correlation

Bodipy fl c16, by Thermo Fisher Scientific (2 mentions) Ultima gold liquid scintillation cocktail, by PerkinElmer (2 mentions)

2 protocols using tri carb 2900tr instrument

1

Retinal Fatty Acid Uptake in Wild-Type and Vldlr Knockout Mice

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We compared retinal long-chain fatty acid uptake of wild type and Vldlr−/− mice gavaged with 0.1 mg of 4,4-difluoro-5,7-dimethyl-4-bora-3a, 4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; Molecular Probes). Mice were euthanized 2 h later and the eyes were enucleated, embedded in OCT and cryo-sectioned (10 µm) for immediate imaging by fluorescence microscopy. Retinal FA uptake was quantitated using 14C-labeled 2-bromopalmitate tracer injected twice daily (i.p.; 0.5 uCi per dose) from P9 to P12; total administered radioactivity was normalized for mouse body weight. Retinas were then dissected and homogenized in Ultima Gold liquid scintillation cocktail (PerkinElmer) and beta-counted (14C DPM) using Tri-Carb 2900TR instrument (PerkinElmer), correcting for background scintillation.
Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.
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2

Retinal Fatty Acid Uptake in Wild-Type and Vldlr Knockout Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
We compared retinal long-chain fatty acid uptake of wild type and Vldlr−/− mice gavaged with 0.1 mg of 4,4-difluoro-5,7-dimethyl-4-bora-3a, 4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; Molecular Probes). Mice were euthanized 2 h later and the eyes were enucleated, embedded in OCT and cryo-sectioned (10 µm) for immediate imaging by fluorescence microscopy. Retinal FA uptake was quantitated using 14C-labeled 2-bromopalmitate tracer injected twice daily (i.p.; 0.5 uCi per dose) from P9 to P12; total administered radioactivity was normalized for mouse body weight. Retinas were then dissected and homogenized in Ultima Gold liquid scintillation cocktail (PerkinElmer) and beta-counted (14C DPM) using Tri-Carb 2900TR instrument (PerkinElmer), correcting for background scintillation.
Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.
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