Aurora a 35c1

Antibodies against EGFR (1005), lamin B (C-20), hnRNP A3 (A15), ERK (K-23), E-cadherin, c-Myc (N-262), cyclin D1 (HD11), and HSP90 were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The antibody against mitochondrial HSP70 (MA3-02) was purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The antibody against COX-2 (ab15191) and aurora A (35C1) were purchased from Abcam (Cambridge, UK). The antibody against β-actin was purchased from Sigma (St. Louis, MO, USA). Alexa Fluor® 594 donkey anti-rabbit IgG (A21207), Alexa Fluor® 647 donkey anti-goat IgG (A21447), and Alexa Fluor® 488 goat anti-mouse IgG (A11001) were purchased from Life Technologies-Molecular Probes.

The effect of alisertib on Aurora A and the Jak/STATs signaling pathways was assessed by immunoblotting, as described previously 21 (link), 23 , using the following phospho-specific antibodies: p-Aurora A/B/C (Cell Signaling Technology, #2914), Aurora A (35C1) (Abcam, ab13824), p-Jak1 (T1022/1023) (Cell Signaling Technology, #3331), Jak1 (6G4) (Cell Signaling Technology, #3344), p-Jak2 (T1007/1008) (Cell Signaling Technology, #3776), Jak2 (D2E12) (Cell Signaling Technology, #3230), p-STAT1 (T701) (Cell Signaling Technology, #7649), STAT1 (D1K9Y) (Cell Signaling Technology, #14994), p-STAT2 (T690) (Cell Signaling Technology, #4441), STAT2 (D9J7L) (Cell Signaling Technology, #72604), PARP (46D11) (Cell Signaling Technology, #9532), DNMT3B (D7070) (Cell Signaling Technology, #67259), DNMT1 (Proteintech., 24206-1-AP), DNMT3A (Proteintech., 19366-1-AP), ubiquitin (Proteintech., 10201-1-AP), UHRF1(Proteintech., 21402-1-AP), flag (Abmart, M20008L) and β-actin (Proteintech.,66009-1-Ig).