Linsmaier skoog medium

A. thaliana ecotype Columbia-0 (Col-0) was used as the wild-type control. The following mutants and transgenic lines, which are in Col-0 background, were used in this study: bzip28–2 (SALK_132285), bzip60–2 (SAIL_283_B03), bzip28–2 bzip60–1 (SALK_132285 SALK_050203), gbf2–1 (SALK_206654), gbf2–2 (SALK_205706), gbf2–3 (SALK_087916), nf-yc2 (SALK_111422), wrky8 (SALK_050194), lbd3 (SAIL_659_D08), anac092 (SALK_090154), anac036 (SAIL_600_D02), hb28 (SALK_096579), erf11 (SALK_085781), nac2 (SALK_037700), rap2.6 (SALK_051006), anac062 (WiscDsLoxHs100_07A), gbf1 (SALK_027691), gbf3 (SALK_056627), GBF2ox (CS2104585) and pGBF2:GBF2-Ypet (CS71581). All T-DNA single and high-order mutants used in this study were confirmed to be homozygous before the analysis. Primers used for genotyping are presented in Table S3. Surface-sterilized seeds were plated on half-strength Linsmaier Skoog (LS) medium (Caisson Labs, Ontario, Canada) supplemented with 1% sucrose (Sigma-Aldrich, St. Louis, MO, USA), and 1.2% Agar (Acumedia, Lansing, MI, USA). Appropriate antibiotics was also added for the screening of transgenic lines. After stratification in the dark at 4 °C for 2 days, plates were transferred to a controlled growth chamber with 80 μmol m−2 s−1 under 16 h light:8 h dark with 22 °C.

Surface-sterilized seeds were plated on half-strength Linsmaier Skoog (LS) medium (Caisson Labs) supplemented with 1% sucrose (Sigma-Aldrich) and 1.2% agar (Acumedia). An appropriate antibiotic was also added for screening of complementation transgenic lines. After stratification in the dark at 4 °C for 2 days, plates were transferred to a controlled growth chamber with 80 μmol m⁻² s⁻¹ under 16 h light:8 h dark at 22 °C.