Dapi reagent

Manufactured by Cell Signaling Technology

DAPI (4',6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize the nuclei of cells.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Glycogen assay kit, by Solarbio (1 mentions) Mitochondrial stress test kit, by Agilent Technologies (1 mentions) Tunel assay kit, by Promega (1 mentions) Cycloheximide (chx), by MedChemExpress (1 mentions) Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Pas staining kit, by Beyotime (1 mentions) Collagenase type 2, by Worthington (1 mentions) Seahorse xf glycolysis stress test kit, by Agilent Technologies (1 mentions) Lactate assay kit, by Nanjing Jiancheng (1 mentions) Ldh assay kit, by Nanjing Jiancheng (1 mentions) Mg132, by MedChemExpress (1 mentions) Jbw301, by Merck Group (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Antifade Reagent with DAPI (5 citations) ProLong™ Gold Antifade reagent containing DAPI (3 citations) Prolong Gold AntiFade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (9 citations) ProLong Gold Antifade Reagent with DAPI (94 citations)

2 protocols using dapi reagent

Metabolic Profiling and Apoptosis Assays in Cell Cultures

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Dulbecco’s modified eagle’s medium (DMEM) and FBS were from Thermo Fisher Scientific. High-sensitivity enhanced chemiluminescence (high-sig ECL) Western blotting substrate was from Tanon. DAPI reagent was from Cell Signaling Technology. Lactate assay kit and LDH assay kit were from Jiancheng Biotech. PAS staining kit was from Beyotime. Glycogen assay kit was from Solarbio. Seahorse XF Glycolysis Stress Test Kit and Mitochondrial Stress Test Kit were from Aglient. The TUNEL assay kit was from Promega. 2-DG, YC-1, CHX, MG132, C646, and propidium iodide (PI) reagents were from MedChemExpress. Sodium Oxa was from Selleckchem. Live/dead viability/cytotoxicity kit was from Thermo Fisher Scientific. Collagenase Type II was from Worthington Biochemical Corporation. Triphenyltetrazolium chloride (TTC) and pancreatin were from Sigma-Aldrich. The antibodies used in this study are listed in Supplemental Table 2.

Yu W., Kong Q., Jiang S., Li Y., Wang Z., Mao Q., Zhang X., Liu Q., Zhang P., Li Y., Li C., Ding Z, & Liu L. (2024). HSPA12A maintains aerobic glycolytic homeostasis and Histone3 lactylation in cardiomyocytes to attenuate myocardial ischemia/reperfusion injury. JCI Insight, 9(7), e169125.

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Quantifying DNA Damage and Repair

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Cells were fixed with 4% paraformaldehyde for 10 minutes at RT, permeabilized with ice cold methanol for 5 minutes at −20°C, and blocked in 3% BSA in PBS for 30 minutes at RT. Cells were then incubated in primary antibodies against RAD51 (Abcam, clone 14B4), or γH2AX (S139, Millipore, clone JBW301). Alexa-Fluor conjugated secondary antibodies were used (Life Technologies). Cell nuclei were stained using DAPI reagent (Cell Signaling Technology Cat. No. 8961). One hundred cells per sample were counted. A cell was counted as γH2AX or RAD51 positive only if >10 foci per nucleus were identified.

Iniguez A.B., Stolte B., Wang E.J., Conway A.S., Alexe G., Dharia N.V., Kwiatkowski N., Zhang T., Abraham B.J., Mora J., Kalev P., Leggett A., Chowdhury D., Benes C.H., Young R.A., Gray N.S, & Stegmaier K. (2018). EWS/FLI confers tumor cell synthetic lethality to CDK12 inhibition in Ewing sarcoma. Cancer cell, 33(2), 202-216.e6.

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