Trypsin lys c mix mass spectrometry

Manufactured by Promega

Trypsin/Lys-C Mix Mass Spectrometry is a laboratory equipment product that facilitates protein digestion for mass spectrometry analysis. It contains a mixture of the proteases trypsin and Lys-C, which are used to cleave proteins into smaller peptide fragments, preparing the samples for mass spectrometric identification and characterization.

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Lab products found in correlation

Vacuum manifold, by Waters Corporation (2 mentions) Spin filter, by Merck Group (2 mentions)

Spelling variants of this product

Trypsin (3007 citations) Trypsin/Lys-C Mix (141 citations) Trypsin/Lys-C (107 citations) Lys-C (104 citations) Trypsin/Lys-C mix mass spectrometry grade (4 citations)

2 protocols using trypsin lys c mix mass spectrometry

Tau Protein Preparation for Mass Spectrometry

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8 M urea, 50 mM Tris–HCl pH 8.5 (100 µl) was added to 20 µl of insoluble Tau fractions. The sample was reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and alkylated with 10 mM chloroacetamide (CAM). The sample was diluted with 100 mM Tris–HCl to a final urea concentration of 2 M and digested overnight with 2 µg Trypsin/Lys-C Mix Mass Spectrometry (1:100 protease:substrate ratio, Promega Corporation). Peptides were desalted on a 50 mg Sep-Pak® Vac (Waters Corporation) employing a vacuum manifold (Waters Corporation). After elution from the column in 70% acetonitrile, 0.1% formic acid (FA), peptides were dried by speed vacuum, resuspended in 40 µL of 0.1% FA, and filtered through a 0.2 µm spin filter (Millipore).

Hallinan G.I., Hoq M.R., Ghosh M., Vago F.S., Fernandez A., Garringer H.J., Vidal R., Jiang W, & Ghetti B. (2021). Structure of Tau filaments in Prion protein amyloidoses. Acta Neuropathologica, 142(2), 227-241.

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Tau Protein Fractionation and Tryptic Digestion

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8 M urea, 50 mM Tris–HCl pH 8.5 (100 μl) was added to 20 μl of insoluble Tau fractions. The sample was reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and alkylated with 10 mM chloroacetamide (CAM). The sample was diluted with 100 mM Tris–HCl to a final urea concentration of 2 M and digested overnight with 2 μg Trypsin/Lys-C Mix Mass Spectrometry (1:100 protease:substrate ratio, Promega Corporation). Peptides were desalted on a 50 mg Sep-Pak® Vac (Waters Corporation) employing a vacuum manifold (Waters Corporation). After elution from the column in 70% acetonitrile, 0.1% formic acid (FA), peptides were dried by speed vacuum, resuspended in 40 μL of 0.1% FA, and filtered through a 0.2 μm spin filter (Millipore).

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