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2 protocols using rm02821

1

Probing Ferroptosis and EMT Mechanisms

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RSL3 (HY-100218, MedChemExpress), chloroquine (CQ) (HY-17589A, MedChemExpress), necrostatin-1 (Nec-1) (HY-15760, MedChemExpress), ferrostatin-1 (Fer-1) (HY-100579, MedChemExpress), SB431542 (HY-10431, MedChemExpress), and carbon 11 (C11)-BODIPY581/591 (RM02821, Abclonal, Wuhan, China) were the reagents used.
Nodal (A9902, Abclonal; sc-373910, Santa Cruz Biotechnology), SCD1 (ab236868, Abcam), Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (8828 S, Cell Signalling Technology), Smad2/3 (ab202445, Abcam; 5678, Cell Signalling Technology), E-cadherin (A20798, Abclonal), N-cadherin (A0432, Abclonal), vimentin (ab8978, Abcam), snail (A5243, Abclonal), GAPDH (A19056, Abclonal), β-tubulin (AC008, Abclonal), goat anti-rabbit immunoglobulin (Ig) G heavy and light chain (H&L) (horse radish peroxidase [HRP]) (ab6721, Abcam), goat anti-mouse IgG (H&L) (HRP) (ab6789, Abcam), IRDye 680 donkey anti-mouse IgG-(H + L)/goat anti-rabbit IRDye 800CW secondary antibody (926-68072/926-32211, LI-COR Biosciences, Lincoln, NE, USA) were the antibodies used in this study.
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2

Boron Dipyrromethene C11 Assay for Hepatocytes

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Hepatocytes were cultured at 37 °C and pretreated with rmGas6 or R428 for 2 h before H/R. The hepatocytes were incubated with boron dipyrromethene C11 (ABclonal, RM02821) at 37 °C for 1 h, and the images were analyzed by measuring the excitation and emission at wavelengths of 460-495 nm and 510-550 nm, respectively, using flow cytometry (Beckman Coulter CytoFlex) and a fluorescence microscope (Olympus DX51, Tokyo, Japan).
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