Soil samples were collected from agricultural areas at different Egyptian governorates. Sampling was done at the subsurface, where about 10 grams of soil samples were suspended in 20 mL of sterile distilled water in sterile 50 mL falcon tubes. Soil suspensions were vortexed and placed in a water bath at 80°C for 30 minutes. Heat-treated suspensions were placed at room temperature for 2 hours, then were serially diluted to 10–1, 10–2 in sterile saline, and were streaked on brain heart agar (Oxoid) plates. Bacilli isolates with different morphology were picked up and isolated for pure colonies on brain heart agar (Oxoid) plates.30
Brain heart agar
Manufactured by Thermo Fisher Scientific
Brain heart agar is a microbiological growth medium used for the cultivation and isolation of a variety of fastidious microorganisms, including streptococci, pneumococci, and Listeria species. It provides nutrients and growth factors necessary for the development of these organisms.
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Lab products found in correlation
1 protocol using brain heart agar
1
Isolation of Bacillus from Egyptian Soils
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