Cx21i light microscope

D-DISH assay was carried out using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay from Ventana Medical Systems. With this, HER2 gene gets detected by a dinitrophenyl (DNP)-labeled probe and visualized using ultraView silver in situ hybridization (SISH) DNP detection Kit. The chromosome 17 centromere is targeted with a digoxigenin (DIG)-labeled probe and detected using ultraView Red ISH DIG detection Kit. On light microscopy, HER2 appears as discrete black signals and chromosome 17 as red signals. The 2-μm-thick sections were loaded into the Ventana Benchmark XT machine. On board, a fully automated procedure was carried out as per the company recommended protocol with the following basic steps of deparaffinization followed by cell conditioning and protease digestion. Subsequently, the probe was applied followed by hybridization and application of the SISH Multimer. This was followed by application of silver chromogen, and then Red ISH Multimer and red chromogen. Finally, counterstain with hematoxylin was followed by clearing in xylene and mounting with dibutylphthalate polystyrene xylene. The slides were evaluated on Olympus CX21i light microscope using the 60× objective lens for better visualization and counting of the signals.

The ultrastructure of fibrin and the distribution of cell bodies within the A-PRF membranes were examined using the Olympus CX21i light microscope (Olympus, New Delhi, India). Before analysis, the A-PRF membranes were fixed in 10% neutral buffered formalin for 24 h at room temperature for paraffin inclusion. 15 (link) The membranes were stored on a microscope slide during fixation to avoid distortion. Successive sections (4-micrometer-thich) were collected along the center of the long axis of the membranes and stained with hematoxylin and eosin (H&E). Each section was divided into 3 areas of equal size, including head/face (proximal), body (center) and tail (distal), as shown in Fig 1C . The center of each area was observed to analyze the distribution of visible cell bodies (marked in dark purple) under ×100, ×200 and ×400 magnification. For semi-quantitative evaluation, manual cell body counting was performed at ×200 magnification by an oral pathologist. The total number of cell bodies counted was used to compare the distribution among the 3 areas of the membrane. 16 (link) Most of the cells were concentrated in the body area.