Lps from e coli 0127 b8 strain

Female C57BL/6 mice received a sublethal or lethal dose of LPS from E. coli (0127: B8 strain, Sigma Aldrich, St. Louis, MO, USA) in a concentration of 20 or 25 mg/kg body weight administrated in sterile PBS by intraperitoneal injection.

The animal protocols used in this work were evaluated and approved by the institutional animal ethics committee (Protocol 9/17 from 28 April 2017) in accordance with Federation for Laboratory Animal Science Associations (FELASA) guidelines. The experiments were performed on outbred white rats. Dams and their pups were kept in cages with a temperature-controlled environment (23 ± 2 °C) with light on from 9 a.m. to 9 p.m. Dams had ad libitum access to food and water, and pups were checked daily for health. The number of pups in a litter was 9–12. Endotoxin-mediated AKI was performed on 3-days-old neonatal and adult rats by intraperitoneal administration of LPS from E. coli (0127:B8 strain, Sigma Aldrich, St. Louis, MO, USA) in concentration 4 mg/kg; the control group was administrated with saline infusion. The total number of animals in each group was 6 (from different litters). The experimental design is presented in Figure 1. Briefly, 24 h after LPS administration, blood, urine and kidneys were taken from animals. Urine samples were diluted 1:1 with 2× sample buffer and boiled for 5 min and then centrifuged at 12,000× g for 3 min. Blood samples were taken to determine blood urea nitrogen (BUN) using AU480 Chemistry System (Beckman Coulter, Brea, CA, USA).