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Glycogen from rabbit liver

Manufactured by Merck Group
Sourced in Australia

Glycogen from rabbit liver is a product designed for laboratory use. It is a polysaccharide molecule extracted from the liver tissue of rabbits, and it serves as a storage form of glucose in the body. This product is intended for research and scientific applications, where its properties and structure may be studied or utilized.

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2 protocols using glycogen from rabbit liver

1

Glycogen and Oil Extraction Protocol

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Oyster glycogen (OG), glycogen from bovine liver (BG), glycogen from rabbit liver (RG) and soybean oil (SO) were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). Phytoglycogen (PG) was purchased from Mirexus, Canada. Vitamin, D, as an oil-soluble nutrient from the brand Blackmore® was purchased from a local pharmacy (Chemist Warehouse, Melbourne, Australia). All solutions were prepared with high purity water (Milli-Q) extracted from a Millipore system with resistivity of 18.2 MΩ/cm at 25 °C.
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2

Maltosyltransferase Activity of GlgE Enzymes

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Maltosyltransferase activities of GlgE proteins of E. lausannensis and W. chondrophila were first evidenced by incubating the purified recombinant enzymes overnight at 30 °C with 10 mg.mL−1 glycogen from rabbit liver (Sigma-Aldrich) and 20 mM orthophosphate in 20 mM Tris-HCl buffer (pH 6.8). The reaction products were separated on thin-layer chromatography Silica gel 60 W (Merck) using the solvent system butanol/ethanol/water (5/4/3 v/v/v) before spraying orcinol (0.2%)-sulfuric (20%) solution to visualize carbohydrates.
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