Fb 1550

The PEO1 (#10032308) and PEO4 (#10032309) cell lines were purchased from the European Collection of Authenticated Cell Cultures, England. The OVCAR3 (#HTB-161) cell line was purchased from American Type Culture Collection, England. PEO1, PEO4 and OVCAR3 cell lines have previously been phenotypically and genotypically characterised as representative of HGSOC [36 (link),37 ]. For routine culture, PEO1 and PEO4 cells were maintained in RPMI 1640 growth medium (#31870, Thermo Fisher Scientific, UK), supplemented with 10% foetal bovine serum (FBS) (#FB-1550, Biosera, USA), 2 mM glutamine (#25030081, Thermo Fisher Scientific, UK), 1% antibacterial antimycotic solution (ABAM) (#A5955, Merck, UK). For routine culture, OVCAR3 cells were maintained in RPMI 1640 growth medium supplemented with 20% FBS (#FB-1550, Biosera, USA), 2 mM glutamine, 0.1% insulin (#I0516, Merck) and 1% ABAM (#A5955, Merck). All cells were cultured in TC treated Corning® T-25 (#CLS430639, Merck), T-75 (#CLS430641U, Merck), or T-175 (#CLS431080, Merck) cell culture flasks and incubated at 37 °C in a humidified 5% CO₂ chamber. Growth medium was replaced every 2–3 days and cells were sub-cultured, using Accutase® cell detachment solution (#SCR005, Sigma–Aldrich), to ensure 70–80% confluency.