Anti atpb clone 3d5

Manufactured by Abcam

Anti-ATPB [clone 3D5] is a monoclonal antibody that recognizes the ATP synthase subunit beta (ATPB) protein. ATPB is a component of the mitochondrial ATP synthase complex, also known as complex V, which is responsible for the final step in oxidative phosphorylation and ATP production. The antibody can be used to detect and study the expression and distribution of ATPB in various biological samples.

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Lab products found in correlation

Anti ulk1 clone d8h5, by Cell Signaling Technology (1 mentions) Anti wipi2, by Bio-Rad (1 mentions) Proteinase inhibitor cocktail, by Merck Group (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions) Ripa buffer, by Merck Group (1 mentions) Ecl substrate, by Bio-Rad (1 mentions)

2 protocols using anti atpb clone 3d5

Immunoblotting and Immunocytochemistry Protocols

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For immunoblotting, the following primary antibodies were used: anti‐ULK1 [clone D8H5] (Cell Signaling Technology, Rabbit MAb, Cat# 8054S, 1:1,000, RRID:AB_11178668), anti‐ATG14 [clone D1A1N] (Cell Signaling Technology, Rabbit MAb, Cat# 96752. 1:1,000, RRID:AB_2737056), pS29 ATG14 [clone D4B8M] (Cell Signaling Technology, Cat# 92340S, 1:1,000, RRID:AB_2800182); anti‐Vinculin [clone EPR 8185], (Abcam, Rabbit MAb, Cat# ab129002; 1:1,000, RRID:AB_2800182. Secondary antibodies for immunoblotting were purchased from Thermo Fisher Scientific. For immunocytochemistry, the following primary antibodies were used: anti‐ATPB [clone 3D5] (Abcam, Cat# ab14730; Mouse mAb, 1:500; RRID:AB_301438); anti‐BNIP3L/Nix [clone D4R4B] (Cell Signaling Technology, Cat# 12396, Rabbit mAb, 1:100; RRID:AB_2688036); anti‐ULK1 [clone D8H5] (Cell Signaling Technology, Rabbit mAb, Cat# 8054S, 1:500, RRID:AB_11178668); anti‐WIPI2 (Bio‐Rad, Cat# MCA5780GA, Mouse mAb, 1:500; RRID:AB_11178668). Secondary antibodies for immunocytochemistry were purchased from Invitrogen (Life Technologies) and used at 1:500. The DNA constructs used (Flag‐ULK1 [DU45617], mCherry‐GFP‐FIS 101‐152 [DU55502]) were generated at the MRC PPU Reagents and Services and are available to purchase online https://mrcppureagents.dundee.ac.uk.

Long M., Sanchez‐Martinez A., Longo M., Suomi F., Stenlund H., Johansson A.I., Ehsan H., Salo V.T., Montava‐Garriga L., Naddafi S., Ikonen E., Ganley I.G., Whitworth A.J, & McWilliams T.G. (2022). DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletion. The EMBO Journal, 41(10), e109390.

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Analyzing AMPK Regulation via Western Blotting

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Cells were lysed with RIPA buffer (Sigma) containing 1 mM PMSF
(Biyuntian) and proteinase inhibitor cocktail (Sigma). The following antibodies
were used: anti-ATP5A (clone 15H4C4, Abcam), anti-ATPB (clone 3D5, Abcam), AMPK
(Cell Signaling), AMPK Pi (Thr172, clone 40H9, Cell Signaling). ECL substrate
(Biorad) was used to detect proteins in Western blotting and the blot bands were
quantified by using Image J.

Jiang W., Le J., Wang P.Y., Cheng X., Smelkinson M., Dong W., Yang C., Chu Y., Hwang P.M., Munford R.S, & Lu M. (2021). Extracellular acidity reprograms macrophage metabolism and innate responsiveness. Journal of immunology (Baltimore, Md. : 1950), 206(12), 3021-3031.

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