Hydrogen
peroxide 30 w/w % was
obtained from Chem-Supply. Ethanol, methanol, and toluene were purchased
from Merck. Dry toluene was prepared by storing toluene over 3 Å
molecular sieves overnight. TEOS, CTAB, Pluronic F127, trimethoxyphenylsilane,
3-mercaptopropyltrimethoxysilane, and 1,3-propane sultone were purchased
from Sigma-Aldrich. CSPTMS in dichloromethane (50 w/w %) was obtained
from Acros Organics. (N,N-Dimethyl-3-aminopropyl)trimethoxysilane
was obtained from Gelest. PBS tablets purchased from AMRESCO were
dissolved in water to obtain PBS pH 7.4 containing 137 mM sodium chloride,
2.7 mM potassium chloride, and 10 mM phosphate buffer. Dulbecco’s
modified Eagle medium (DMEM) was obtained from Invitrogen and was
supplemented with penicillin,l -glutamine, and either 2 or
10 v/v % fetal calf serum (FCS). The HSV-1 used was the HSV-1 H129-GFP
strain described in McGovern et al.39 (link) HSV-2
was a clinical isolate. Acyclovir was purchased from TCI. Unless stated
otherwise, the experiments were conducted at room temperature (ca.
23° C). The water used in this study was type II pure water according
to ASTM specification D1193-06.
peroxide 30 w/w % was
obtained from Chem-Supply. Ethanol, methanol, and toluene were purchased
from Merck. Dry toluene was prepared by storing toluene over 3 Å
molecular sieves overnight. TEOS, CTAB, Pluronic F127, trimethoxyphenylsilane,
3-mercaptopropyltrimethoxysilane, and 1,3-propane sultone were purchased
from Sigma-Aldrich. CSPTMS in dichloromethane (50 w/w %) was obtained
from Acros Organics. (N,N-Dimethyl-3-aminopropyl)trimethoxysilane
was obtained from Gelest. PBS tablets purchased from AMRESCO were
dissolved in water to obtain PBS pH 7.4 containing 137 mM sodium chloride,
2.7 mM potassium chloride, and 10 mM phosphate buffer. Dulbecco’s
modified Eagle medium (DMEM) was obtained from Invitrogen and was
supplemented with penicillin,
10 v/v % fetal calf serum (FCS). The HSV-1 used was the HSV-1 H129-GFP
strain described in McGovern et al.39 (link) HSV-2
was a clinical isolate. Acyclovir was purchased from TCI. Unless stated
otherwise, the experiments were conducted at room temperature (ca.
23° C). The water used in this study was type II pure water according
to ASTM specification D1193-06.