Genomic DNA was extracted from frozen tissue using the QIAamp DNA mini kit (Qiagen, Hilden, Germany). Cryosections were prepared prior to DNA isolation to guarantee tumor cell content. DNA Exome libraries were prepared using the Nextera Rapid Capture Enrichment Kit, CEX version (Coding Exome Oligos; Illumina, San Diego, USA). Sequencing was performed on a Hiseq4000 instrument (Illumina) with 1% phiX (v3, Illumina) spike-in at 2*75 bp paired-end settings with the 150 bp SBS chemistry. The purity- and ploidy status of the discovery cohort (per case) are shown in Additional file 2 : Table S1. Sequencing statistics are summarized in Additional file 2 : Table S2.
Nextera rapid capture enrichment kit cex version coding exome oligos
Manufactured by Illumina
Sourced in United States
The Nextera Rapid Capture Enrichment Kit, CEX version (Coding Exome Oligos) is a targeted enrichment solution designed for efficient and rapid capture of the coding exome regions of the human genome. The kit utilizes Illumina's Nextera library preparation technology and custom-designed capture probes to enable selective sequencing of the coding exome.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using nextera rapid capture enrichment kit cex version coding exome oligos
1
Whole-Exome Sequencing of Tumor Samples
2
Tumor DNA Exome Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Genomic DNA was extracted from frozen tissue using the QIAamp DNA mini kit (Qiagen, Hilden, Germany). Cryosections were prepared prior to DNA isolation to guarantee tumor cell content. DNA Exome libraries were prepared using the Nextera Rapid Capture Enrichment Kit, CEX version (Coding Exome Oligos; Illumina, San Diego, USA). Sequencing was performed on a Hiseq4000 instrument (Illumina) with 1% phiX (v3, Illumina) spike-in at 2*75 bp pairedend settings with the 150bp SBS chemistry.
3
Tumor DNA Exome Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genomic DNA was extracted from frozen tissue using the QIAamp DNA mini kit (Qiagen, Hilden, Germany). Cryosections were prepared prior to DNA isolation to guarantee tumor cell content. DNA Exome libraries were prepared using the Nextera Rapid Capture Enrichment Kit, CEX version (Coding Exome Oligos; Illumina, San Diego, USA). Sequencing was performed on a Hiseq4000 instrument (Illumina) with 1% phiX (v3, Illumina) spike-in at 2*75 bp pairedend settings with the 150bp SBS chemistry.
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