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Fitc labeled annexin 5 and pi

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FITC-labeled Annexin V and PI are fluorescently-labeled proteins used for detecting and quantifying apoptosis, a type of programmed cell death. Annexin V binds to phosphatidylserine, which is exposed on the cell surface during apoptosis. FITC (Fluorescein Isothiocyanate) is used to label Annexin V, allowing for detection by fluorescence microscopy or flow cytometry. Propidium Iodide (PI) is a DNA-intercalating dye that can be used to identify late-stage apoptotic or necrotic cells.

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Facscalibur machine, by BD (1 mentions) Annexin 5 fitc pi, by Keygen Biotech (1 mentions)

3 protocols using fitc labeled annexin 5 and pi

1

Annexin V/PI Apoptosis Assay

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After the indicated treatments, cells were stained by FITC-labeled Annexin V and PI (Nanjing Keygen Biotech, Nanjing, Jiangsu, China) depending to its protocol. FACS analysis for Annexin V/PI double staining was done with a BD FACS Calibur machine (BD Biosciences). Dead cells consisted of PI+ cells (Q1), PI + FITC+ cells (Q2) and FITC+ cells (Q4). All experiments were carried out three times.
Zhang D., Zhao Q., Sun H., Yin L., Wu J., Xu J., He T., Yang C, & Liang C. (2016). Defective autophagy leads to the suppression of stem-like features of CD271+ osteosarcoma cells. Journal of Biomedical Science, 23, 82.
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2

Annexin V-FITC and PI Apoptosis Assay

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BV2 cells were seeded on 6‐well plates for 24 h. After being treated with OGD and Met, the cells were harvested and suspended in PBS, and then treated for 30 min with FITC‐labeled Annexin V and PI (Nanjing KeyGen Biotech. Co. Ltd.) for 10 min at room temperature in the dark. Next, the cells were incubated with binding buffer (400 μl). Finally, the flow cytometry (EMD Millipore, Billerica, MA, USA) was used to test cell apoptosis.
Ruan C., Guo H., Gao J., Wang Y., Liu Z., Yan J., Li X, & Lv H. (2021). Neuroprotective effects of metformin on cerebral ischemia‐reperfusion injury by regulating PI3K/Akt pathway. Brain and Behavior, 11(10), e2335.
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3

Annexin V-FITC/PI Assay for Paclitaxel-Treated MCF-7 Cells

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For the annexin V-FITC/PI assay, MCF-7/paclitaxel cells were collected after a 72-h treatment and stained with FITC-labeled annexin V and PI according to the manufacturer's instructions (KeyGEN Biotech Inc., China). The fluorescence levels were analyzed by flow cytometry (Beckman Coulter, USA).
Mei M., Xie D., Zhang Y., Jin J., You F., Li Y., Dai J, & Chen X. (2014). A New 2α,5α,10β,14β-tetraacetoxy-4(20),11-taxadiene (SIA) Derivative Overcomes Paclitaxel Resistance by Inhibiting MAPK Signaling and Increasing Paclitaxel Accumulation in Breast Cancer Cells. PLoS ONE, 9(8), e104317.
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