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Mgieasy fs dna library prep kit

Manufactured by Covaris
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The MGIEasy FS DNA Library Prep kit is a laboratory equipment product designed for the preparation of DNA libraries. The kit provides the necessary reagents and protocols for constructing DNA libraries from various sample types, including genomic DNA and amplified DNA. The core function of the kit is to enable the generation of DNA libraries suitable for next-generation sequencing analysis.

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Lab products found in correlation

E220 evolution focused ultrasonicator, by Covaris (2 mentions)

2 protocols using mgieasy fs dna library prep kit

1

Whole Genome Sequencing Library Prep

Check if the same lab product or an alternative is used in the 5 most similar protocols
For small insert-size libraries, genomic DNA was sheared (300~500-bp) with the Covaris E220 Evolution Focused-Ultrasonicator (Covaris, Inc., Woburn, MA) and subjected for library construction using the MGIEasy FS DNA Library Prep kit according to the manufacturer’s protocol. Libraries were pooled and sequenced to paired-end 100-bp with a read-depth of ~4-fold for each sample on an MGISEQ-2000 platform (MGI Tech Co., Ltd., Shenzhen, China).
For mate-pair libraries, 1-μg of genomic DNA was sheared (3~8-kb) by a HydroShear device (Digilab, Inc., Hopkinton, MA)22 (link), and subjected for library construction as previously described (Supplementary methods)28 . Samples were pooled and sequenced on an MGISEQ-2000 platform (MGI) for a read-depth of ~4-fold (paired-end 100-bp) for each sample.
Dong Z., Chau M.H., Zhang Y., Yang Z., Shi M., Wah Y.M., Kwok Y.K., Leung T.Y., Morton C.C, & Choy K.W. (2021). Low-pass genome sequencing-based detection of absence of heterozygosity: validation in clinical cytogenetics. Genetics in medicine : official journal of the American College of Medical Genetics, 23(7), 1225-1233.
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2

Whole Genome Sequencing Library Prep

Check if the same lab product or an alternative is used in the 5 most similar protocols
For small insert-size libraries, genomic DNA was sheared (300~500-bp) with the Covaris E220 Evolution Focused-Ultrasonicator (Covaris, Inc., Woburn, MA) and subjected for library construction using the MGIEasy FS DNA Library Prep kit according to the manufacturer’s protocol. Libraries were pooled and sequenced to paired-end 100-bp with a read-depth of ~4-fold for each sample on an MGISEQ-2000 platform (MGI Tech Co., Ltd., Shenzhen, China).
For mate-pair libraries, 1-μg of genomic DNA was sheared (3~8-kb) by a HydroShear device (Digilab, Inc., Hopkinton, MA)22 (link), and subjected for library construction as previously described (Supplementary methods)28 . Samples were pooled and sequenced on an MGISEQ-2000 platform (MGI) for a read-depth of ~4-fold (paired-end 100-bp) for each sample.
Dong Z., Chau M.H., Zhang Y., Yang Z., Shi M., Wah Y.M., Kwok Y.K., Leung T.Y., Morton C.C, & Choy K.W. (2021). Low-pass genome sequencing-based detection of absence of heterozygosity: validation in clinical cytogenetics. Genetics in medicine : official journal of the American College of Medical Genetics, 23(7), 1225-1233.
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