Stool collection containers were distributed by healthcare professionals, and participating individuals provided one fresh morning stool sample, which was transferred to the local hospital laboratory in Dabou. On the day of collection, the stool samples were examined microscopically by two senior laboratory technicians for the presence of a broad range of parasitic infections. Specifically, the following tests were employed: the Kato-Katz method (duplicate Kato-Katz thick smears for the diagnosis of S. mansoni and soil-transmitted helminths); the formalin-ether concentration technique (FECT) (for the diagnosis of helminths and intestinal protozoa); and Baermann funnel concentration and Koga agar plate culture (for the diagnosis of S. stercoralis and hookworm). Additionally, for the diagnosis of G. intestinalis and Cryptosporidium species, an antigen detection RDT was employed (Crypto/Giardia Duo-Strip; Coris BioConcept, Gembloux, Belgium). At the time of the study, the performance of bacterial stool culture was logistically not feasible at Dabou hospital.
Crypto giardia duo strip
Manufactured by BioConcept
Sourced in Belgium
The Crypto/Giardia Duo-Strip is a diagnostic device used for the simultaneous detection of Cryptosporidium and Giardia antigens in stool samples. It utilizes an immunochromatographic assay technology to provide qualitative results.
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Lab products found in correlation
3 protocols using crypto giardia duo strip
1
Multiplex Parasitic Infection Screening
2
Evaluation of Commercial Rapid Diagnostic Tests for Giardia and Cryptosporidium
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Three different commercial kits were evaluated: GIARDIA/CRYPTOSPORIDIUM QUIK-CHEK (Produced by TechLab, USA; Lot: 1016037; expiration date 1 April 2018); RIDA-QUICK for Cryptosporidium/Giardia Combi (Produced by R-Biopharm, Germany; Lot: M52.37; expiration date: May 2018); CRYPTO/GIARDIA DUO-Strip (Produced by Coris Bio Concept, Belgium; Lot: 36464 L1602; expiration date: 15 November 2017).
The assays were conducted at room temperature exclusively on stool samples. All tests were performed according to the manufacturer’s instructions. The diagnostic evaluation was performed on all from the two trial sites in Kenya (n = 120) and a selection of samples from the Malawi site (n = 116), as not all collected samples from Malawi were of sufficient quantity to perform all three RDT tests. In total 236 stool samples were tested with all three RDTs. The RDTs were read by two independent readers who were blinded from the PCR results and in case of discordance (positive vs negative) a third independent reader was consulted whose conclusion was decisive. Test performance was reported in terms of sensitivity and specificity using the multiplex real-time PCR results as reference standard.
The assays were conducted at room temperature exclusively on stool samples. All tests were performed according to the manufacturer’s instructions. The diagnostic evaluation was performed on all from the two trial sites in Kenya (n = 120) and a selection of samples from the Malawi site (n = 116), as not all collected samples from Malawi were of sufficient quantity to perform all three RDT tests. In total 236 stool samples were tested with all three RDTs. The RDTs were read by two independent readers who were blinded from the PCR results and in case of discordance (positive vs negative) a third independent reader was consulted whose conclusion was decisive. Test performance was reported in terms of sensitivity and specificity using the multiplex real-time PCR results as reference standard.
3
Intestinal Helminth and Protozoan Infections
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Intestinal helminth infections were determined in morning stool samples using the Kato‐Katz technique and evaluated as described previously.30 According to the World Health Organization (WHO) classification, infection intensity with the soil‐transmitted helminths (STH) Ascaris lumbricoides (A. lumbricoides) and Trichuris trichiura (T. trichiura) was based on the number of eggs per gram (EPG) of stool.31 Additionally, stool samples were screened for the protozoans Cryptosporidium spp. and Giardia intestinalis using the Crypto‐Giardia Duo Strip® rapid diagnostic test (Coris, BioConcept, Gembloux, Belgium).
For more details on parasitological examinations, seeSupplement .
For more details on parasitological examinations, see
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