Pegylated liposomal doxorubicin

Manufactured by Johnson & Johnson

Sourced in Belgium

Pegylated liposomal doxorubicin is a type of drug delivery system. It consists of the chemotherapeutic agent doxorubicin encapsulated within a liposomal formulation that has been modified with polyethylene glycol (PEG) to prolong its circulation time in the body.

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Lab products found in correlation

Doxil, by Johnson & Johnson (2 mentions) Doxorubicin hydrochloride, by Merck Group (1 mentions) Tgf β1, by R&D Systems (1 mentions) Gemzar, by Eli Lilly (1 mentions) Exo spin exosome purification kit, by Cell Guidance Systems (1 mentions) Cyquant ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Capricorn (1 mentions) Paclitaxel, by Fujifilm (1 mentions) Human immunoglobulin g huigg, by MP Biomedicals (1 mentions) Cholesterol chol, by Merck Group (1 mentions) Doxorubicin, by Teva Pharmaceuticals (1 mentions)

5 protocols using pegylated liposomal doxorubicin

Solubilization and Preparation of Experimental Compounds

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For in vivo studies, tranilast (Rizaben, Kissei Pharmaceutical, Japan) was solubilized with 1% NaHCO₃ followed by heating at 70 °C for 1 h (33.3 mg/ml), as previously described36 (link)37 (link). For in vitro experiments, tranilast was dissolved with DMSO at 100 mM stock concentration and TGFβ1 (R&D systems) was dissolved with sterile 4 mM HCl containing 1 mg/ml BSA at 1 μg/ml stock concentration. Doxorubicin hydrochloride (Sigma) was dissolved (625 μg/ml stock) in phosphate buffer saline (PBS). Doxil (Pegylated liposomal doxorubicin, Janssen Pharmaceuticals) was purchased as already made solution (2 mg/ml) and Abraxane (Albumin-bound paclitaxel, Celgene) was solubilized in 0.9% NaCl in final stock concentration of 5 mg/ml.

Papageorgis P., Polydorou C., Mpekris F., Voutouri C., Agathokleous E., Kapnissi-Christodoulou C.P, & Stylianopoulos T. (2017). Tranilast-induced stress alleviation in solid tumors improves the efficacy of chemo- and nanotherapeutics in a size-independent manner. Scientific Reports, 7, 46140.

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Formulation and Preparation of Anticancer Drugs

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Vismodegib (GDC-0449, Erivedge) was purchased from Selleckchem and the compound was formulated in a 10 mg/mL suspension in MCT (0.5% methylcellulose, 0.2% Tween 80), as previously described [16 (link)]. Gemzar (Gemcitabine, Lilly) was dissolved in 0.9% NaCl (12.5 mg/ml stock). Doxil (Pegylated liposomal doxorubicin, Janssen Pharmaceuticals) was purchased as already made solution (2 mg/ml) and Abraxane (Albumin-bound paclitaxel, Celgene) was solubilized in 0.9% NaCl in final stock concentration of 5 mg/ml.

Mpekris F., Papageorgis P., Polydorou C., Voutouri C., Kalli M., Pirentis A.P, & Stylianopoulos T. (2017). Sonic-Hedgehog pathway inhibition normalizes desmoplastic tumor microenvironment to improve chemo- and nanotherapy. Journal of controlled release : official journal of the Controlled Release Society, 261, 105-112.

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Exosome Purification and Characterization

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PEGylated liposomal doxorubicin was obtained from Janssen (Belgium). Exo-spin™ exosome purification kit was obtained from Cell Guidance systems (UK). CyQUANT™ LDH cytotoxicity assay kit and 3.9-μm latex beads were purchased from Invitrogen (USA). Exosome-free fetal bovine serum (FBS) was from System Biosciences (USA) and RPMI 1640 was from Capricorn (Germany). BCA protein assay kit was purchased from Parstous Biotechnology (Iran). Anti-CD63/PE antibody and Isotype control mouse IgG/PE was from BioLegend (USA). Annexin V-FITC/PI kit was obtained from MabTag (Germany).

Hekmatirad S., Moloudizargari M., Moghadamnia A.A., Kazemi S., Mohammadnia-Afrouzi M., Baeeri M., Moradkhani F, & Asghari M.H. (2021). Inhibition of Exosome Release Sensitizes U937 Cells to PEGylated Liposomal Doxorubicin. Frontiers in Immunology, 12, 692654.

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Combination Therapy Dosage Protocols

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Bevacizumab was manufactured by Chugai Pharmaceutical Co., Ltd. (Tokyo, Japan) as a solution and diluted to 5 mg/kg body weight (0.2 ml/mouse) with saline. Human immunoglobulin G (HuIgG) was purchased from MP Biomedicals, LLC (Santa Ana, CA, USA), and was reconstituted with water and diluted to 5 mg/kg body weight (0.2 ml/mouse) with saline (Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan). Paclitaxel was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) and was reconstituted with 50% ethanol-50% Cremophor EL, and immediately prior to administration was diluted 10-fold with saline. PEGylated liposomal doxorubicin was purchased from Janssen Pharmaceutical, Inc. (Beerse, Belgium) as a solution and diluted to 20 mg/kg body weight (10 ml/kg body weight) with saline.

Ishikura N., Yorozu K., Kurasawa M., Yanagisawa M., Sugimoto M, & Yamamoto K. (2019). Sustained effect of continuous treatment with bevacizumab following bevacizumab in combination with chemotherapy in a human ovarian clear cell carcinoma xenograft model. Oncology Reports, 42(3), 1057-1065.

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Liposomal Drug Delivery Formulations

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The following components
were used: hydrogenated soybean phosphatidylcholine (HSPC) (Lipoid,
Germany), methoxypolyethylene glycol distearoyl phosphatidylethanolamine
(mPEG2000-DSPE) (Biolab, Jerusalem, Israel), cholesterol (Chol) (Sigma,
St. Louis, MO), alendronic acid (Teva, Israel, and Tokyo Chemical
Industry Co Ltd., Japan), doxorubicin (Teva, Israel), PEGylated liposomal
doxorubicin (Janssen Pharmaceuticals, NJ, USA, commercial name Doxil
or Caelyx), mitomycin C prodrug (MLP) conjugate: 2,3-(distearoyloxy)propane-1-dithio-4′-benzyloxycarbonyl-MMC
(Aptuit-Laurus, Hyderabad, India).

Edmonds S., Volpe A., Shmeeda H., Parente-Pereira A.C., Radia R., Baguña-Torres J., Szanda I., Severin G.W., Livieratos L., Blower P.J., Maher J., Fruhwirth G.O., Gabizon A, & T. M. de Rosales R. (2016). Exploiting the Metal-Chelating Properties of the Drug Cargo for In Vivo Positron Emission Tomography Imaging of Liposomal Nanomedicines. ACS Nano, 10(11), 10294-10307.

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