The paraffin section was stained with hematoxylin and eosin and Masson’s trichrome. To detect neovessels and myofibroblasts, rabbit anti-mouse CD31 antibody (30-times dilution: Dianova GmbH, Hamburg, Germany) and rabbit anti-mouse α-smooth muscle cell actin (α-SMA) antibody (3000-times dilution: Abcam plc, Tokyo, Japan) were used, respectively. After treatment with an enhancing reagent (EnVision Kit, DAKO Japan, Inc., Tokyo, Japan), immunohistologic localization of α-SMA was visualized by 3,3’-diaminobenzidine. Hematoxylin was used for counterstaining.
Rabbit anti mouse cd31 antibody
Manufactured by Dianova
Sourced in Germany
Rabbit anti-mouse CD31 antibody is a laboratory reagent used in immunochemical techniques. It binds specifically to the CD31 (PECAM-1) protein expressed on the surface of mouse endothelial cells. This antibody can be utilized for the identification and analysis of endothelial cells in various research applications.
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Lab products found in correlation
2 protocols using rabbit anti mouse cd31 antibody
1
Immunohistochemical Analysis of Angiogenesis
2
Wound Healing Histological Analysis
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On days 0, 7, 14, 21, and 87, the mice were sacrificed under an overdose of general
anesthesia, and the abdominal wall, including the wound tissue, was excised and fixed in
10% buffered formalin (Sigma-Aldrich Japan), and paraffin blocks were prepared. The center
of the wound cross-section was determined by sectioning the wound tissue embedded in
paraffin. Ten serial sections of 4-μm thickness were initially prepared for staining with
hematoxylin–eosin (H&E) stain, Masson’s trichrome (MT) stain, and
immunohistochemistry. To detect neovessels and myofibroblasts, rabbit anti-mouse CD31
antibody (Dianova GmbH, Hamburg, Germany) and rabbit anti-mouse α-smooth muscle actin
(SMA) antibody (Abcam plc, Tokyo, Japan), respectively, were used. After treatment with an
enhancing reagent (EnVision Kit, DAKO Japan, Inc., Tokyo, Japan), immunohistochemical
localization of α-SMA was visualized using 3,3′-diaminobenzidine. Hematoxylin was used for
counterstaining. For collagen distribution, paraffin sections were stained with 0.1%
Sirius red in saturated picric acid solution for 1 hr and examined by polarization
microscopy.
anesthesia, and the abdominal wall, including the wound tissue, was excised and fixed in
10% buffered formalin (Sigma-Aldrich Japan), and paraffin blocks were prepared. The center
of the wound cross-section was determined by sectioning the wound tissue embedded in
paraffin. Ten serial sections of 4-μm thickness were initially prepared for staining with
hematoxylin–eosin (H&E) stain, Masson’s trichrome (MT) stain, and
immunohistochemistry. To detect neovessels and myofibroblasts, rabbit anti-mouse CD31
antibody (Dianova GmbH, Hamburg, Germany) and rabbit anti-mouse α-smooth muscle actin
(SMA) antibody (Abcam plc, Tokyo, Japan), respectively, were used. After treatment with an
enhancing reagent (EnVision Kit, DAKO Japan, Inc., Tokyo, Japan), immunohistochemical
localization of α-SMA was visualized using 3,3′-diaminobenzidine. Hematoxylin was used for
counterstaining. For collagen distribution, paraffin sections were stained with 0.1%
Sirius red in saturated picric acid solution for 1 hr and examined by polarization
microscopy.
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