CD8+ T cells were isolated from the TGs of VEGF-Aflox/flox mice at day 7 pi using the MACS column-based CD8a T cell isolation kit (Miltenyibiotec). Approximately 5 × 105 isolated CD8+ T cells were then cultured in a 24-well plate in the presence or absence of PMA/Ionomycin (EMD Millipore) or DMSO (Sigma-Aldrich) vehicle as previously described49 . The cells were then harvested and intracellularly stained with anti-interferon-γ APC (eBioscience, Catalogue # 17-7311) followed by surface stain for CD3, CD8, and CD45, antigen expression 3 hr post-stimulation.
Anti interferon γ apc
Manufactured by Thermo Fisher Scientific
The Anti-interferon-γ APC is a labelled antibody used for the detection and quantification of interferon-gamma in cell-based assays and flow cytometry applications. The antibody is conjugated with the fluorescent dye Allophycocyanin (APC), which allows for the visualization and analysis of interferon-gamma-producing cells.
Automatically generated - may contain errors
2 protocols using anti interferon γ apc
1
Interferon-γ Production in CD8+ T Cells
2
Interferon-γ Production in CD8+ T Cells
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