Dba 2j

Specific pathogen-free female DBA/2J, ICR, C57BL/6J, and BALB/cA mice (6 weeks old) were purchased from CLEA Japan, Inc. (Tokyo, Japan), and A/JJms, C3H/HeJYok and ddY mice (6 weeks old) were obtained from Japan SLC, Inc. (Hamamatsu, Shizuoka, Japan). The animals were housed plastic cages in an air-conditioned room at 23 ± 2°C with a relative humidity of 55 ± 10% under a 12-hour light/dark cycle, fed a standard laboratory diet and given water ad libitum. Animal experiments were approved by the Institutional Animal Care and Use Committee for Kitasato University and performed in accordance with Guide for the Care and Use of Laboratory Animals in the Kitasato University and the National Research Council Guide for the Care and Use of Laboratory Animals in Japan.

All animal experiments were approved by the Animal Research Committee of the RIKEN BioResource Center and were performed in accordance with RIKEN guidelines for animal experiments. Three laboratory mouse strains, C57BL/6J, C3H/HeJ, and DBA/2J, were purchased from a commercial breeder (CLEA Japan, Inc., Japan) and maintained in our facility (RIKEN BioResource Center Research Building for Animal Models of Human Disease). All mice were maintained at constant room temperature (23 °C ± 2 °C) and humidity (55 ± 10%) under a light/dark cycle of 12/12 h in a specific pathogen-free (SPF) environment. The pathogens were specified in categories A and B of the International Council for Laboratory Animal Science. Mice were weaned between the ages of 4 and 5 weeks. Mice had ad libitum access to water and conventional chow diet, CA-1 (CLEA Japan, Inc., Japan).