Biotinylated wfa

Manufactured by Merck Group

Biotinylated WFA is a lectin-based reagent derived from Wisteria floribunda. It is labeled with biotin, a small molecule that can be used to detect the lectin through various detection methods.

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Lab products found in correlation

Avidin biotinylated horseradish peroxidase solution abc, by Vector Laboratories (1 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions) Rabbit anti parvalbumin, by Abcam (1 mentions) Hoechst, by Thermo Fisher Scientific (1 mentions) Chabc, by Merck Group (1 mentions) Rabbit anti myc tag, by OriGene (1 mentions) Chicken anti neun, by Merck Group (1 mentions) Mouse anti 6x his tag, by Abcam (1 mentions)

2 protocols using biotinylated wfa

Histochemical Localization of WFA

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Sections were collected in 0.05M Tris buffered saline (TBS), preincubated in 0.05M TBS with 0.5% Triton-X (TBS-X) (90–120 minutes, with rocking), and incubated overnight at room temperature in biotinylated WFA (Sigma, St. Louis, MO) in TBS-X (1:200). After rinsing, sections were transferred to avidin biotinylated horseradish peroxidase solution (ABC; Vector Labs, Burlingame, CA) for 3–5 hours (room temperature), and, after further rinsing, finally reacted in 0.55 mM 3–3’ diaminobenzidine (DAB; Sigma, St. Louis, MO) with H₂O₂ (26μl per 80ml).

Pletikos M, & Rockland K.S. (2018). WFA-labeled Perineuronal Nets in the Macaque Claustrum. Claustrum, 3(1), 1536104.

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Comprehensive Neuronal Immunostaining Protocol

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For immunohistochemistry, virus injection, neuronal cultures and Western Blots, the following are used: goat anti-Nptx2 (Santa Cruz, sc-12125), chicken anti-NeuN (Millipore, abn91), rabbit anti-myc-tag (OriGene, TA100029), rabbit anti-parvalbumin (Abcam, ab11427), biotinylated WFA (Sigma-Aldrich, L1516), mouse anti-6X his-tag (Abcam, ab18184), Hoechst (Thermo Fisher, H3570), his-tag NPTX2 protein (R&D Systems, 10889), chABC (Sigma-Aldrich, C3667), test hyase (Sigma-Aldrich, H3506), strep hyase (Sigma-Aldrich, H1136), and lenti-cmv-Nptx2 lentivirus (10⁷ TU/mL, OriGene, MR206833L1V).

Van't Spijker H.M., Rowlands D., Rossier J., Haenzi B., Fawcett J.W, & Kwok J.C. (2019). Neuronal Pentraxin 2 Binds PNNs and Enhances PNN Formation. Neural Plasticity, 2019, 6804575.

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