The bacterial strain and plasmids involved in this study are listed in Table 1 . E. coli TOP10 was used as the host, and cultured in M9 medium (0.68% Na2HPO4, 0.3% KH2PO4, 0.05% NaCl, 0.1% NH4Cl, 0.01% MgSO4, and 0.001% CaCl2) supplemented with glucose at 0.4%, IPTG at 0.1 mM, and ampicillin at 50 μg/mL. Stock solutions of CaCl2, MgCl2, FeSO4, MnSO4, NiSO4, CuSO4, ZnSO4, CdCl2, Pb(NO3)2, and HgCl2 were freshly prepared with analytical grade chemicals and distilled water. SP sepharose fast flow was obtained from GE Healthcare (USA). Isopropyl-β-D-thiogalactopyranoside (IPTG) and o-nitrophenyl-β-D-galactopyranoside (ONPG) were obtained from Sangon Biotech (Shanghai, China). All oligonucleotide primers and DNA fragments were synthesized by Sangon Biotech (Shanghai, China).
O nitrophenyl β d galactopyranoside onpg
Manufactured by Sangon
Sourced in China
O-nitrophenyl-β-D-galactopyranoside (ONPG) is a synthetic substrate used for the detection and quantification of β-galactosidase enzyme activity. It is a colorless compound that, upon hydrolysis by β-galactosidase, releases a yellow-colored o-nitrophenol product, which can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using o nitrophenyl β d galactopyranoside onpg
1
Bacterial Strain Cultivation and Heavy Metal Biosorption
2
Heterologous Lactase Production in Pichia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Escherichia coli strain JM109 and Pichia pastoris X33 were used as gene cloning and expression hosts, respectively. The vector pPICZαA was used as the expression vector. The lacA gene [GenBank (https: / / www .ncbi .nlm .nih .gov/ genbank/ ) number: XP_001727461.1] was optimized in accordance with the codon preference of P. pastoris and synthesized by Sangon. The yeast extract peptone dextrose medium (YPD), buffered glycerolcomplex medium (BMGY), and buffered methanolcomplex medium (BMMY) were prepared according to the Multi-Copy Pichia Expression Kit (Invitrogen) for yeast growth and protein production. o-Nitrophenylβ-d-galactopyranoside (oNPG) and o-nitrophenol were obtained from Sangon. The Glucose Assay Kit was purchased from Megazyme. The UHT milk was purchased from Mengniu Dairy Co. Ltd. Other chemicals and reagents not mentioned were of reagent grade, purchased from Sinopharm Chemical Reagent Co. Ltd. The commercial lactase used for milk lactose hydrolysis was kindly provided from Sunsonenzymes Co. Ltd.
No animals were used in this study, and ethical approval for the use of animals was thus deemed unnecessary.
No animals were used in this study, and ethical approval for the use of animals was thus deemed unnecessary.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!