Mouse anti-filamin (F6682) antibody was purchased from Sigma-Aldrich. Rabbit anti-p65 (sc-109) and mouse anti-EPLIN (sc-136399) antibodies were from Santa Cruz Biotechnology. Mouse anti-ADAMDEC1 antibody was kindly provided by Drs. Lund and Petersen (Novo Nordisk A/S)29 (link). For immunofluorescence, the primary antibodies were diluted in phosphate-buffered saline (PBS) containing 1% bovine serum albumin (BSA) at 1:100. Alexa-Fluor-568- and -647-conjugated secondary antibodies (Life Technologies) were used at 1:200. Alexa-Fluor-568- and -647-conjugated phalloidin (Life Technologies) was used at 1.0 U ml−1. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. Stable isotope-labeled amino acids were purchased from Cambridge Isotope Laboratories. The synthetic peptide containing stable isotope labeled phenylalanine (SLNSPEQEDF[13C9,15N]LQAEK) was obtained from SCRUM Inc.
Mouse anti filamin f6682 antibody
Manufactured by Merck Group
Sourced in United Kingdom
The Mouse anti-filamin (F6682) antibody is a laboratory reagent used in research applications. It specifically targets and binds to the filamin protein, which plays a role in the organization and dynamics of the actin cytoskeleton. The antibody can be used in various immunoassay techniques to detect and study the filamin protein.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 568 and 647 conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions)
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Type 1 collagen cellmatrix type 1 a, by Nitta Gelatin (1 mentions)
Alexa fluor 568 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)
Sphingosine 1 phosphate (s1p), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using mouse anti filamin f6682 antibody
1
Immunofluorescence of Cytoskeletal Proteins
2
Immunofluorescence Staining of Filamin
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Mouse anti-filamin (F6682) antibody was from Sigma-Aldrich (Gillingham, United Kingdom). Alexa Fluor 568–conjugated phalloidin (Life Technologies, Paisley, United Kingdom) was used at 1.0 U/ml. Alexa Fluor 568– and 647–conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5000. For immunofluorescence, primary antibody was used at 1:100, and all secondary antibodies were used at 1:200. Type I collagen (Cellmatrix Type I-A) was obtained from Nitta Gelatin (Osaka, Japan) and neutralized on ice to a final concentration of 2 mg/ml according to the manufacturer’s instructions. S1P, SPC, and bovine serum albumin (BSA) were from Sigma-Aldrich.
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