Fuji dri chem slide kit

At 24 h after thromboembolism, the rats were deeply anesthetized via an intraperitoneal injection of 4% chloral hydrate (10 ml/kg). Blood samples were collected from the axillary vein.
Adverse drug reactions such as renal and hepatic disorders are occasionally observed during edaravone treatment in >5% of patients [27 (link)]. To evaluate adverse drug reactions including renal and hepatic disorders, the serum aspartate transaminase, serum alanine transaminase, blood urea nitrogen, and serum creatinine levels were measured by an enzymatic method using a Fuji DRI-CHEM Slide Kit (Fujifilm Medical, Tokyo, Japan).

For serum biochemical analysis, whole blood was collected from the heart. Among the 10 samples per group, two samples were dropped out of the blood collection process, the eight samples were used for further analysis. Serum was collected by centrifugation of blood samples at 3000 rpm for 10 min at 4 °C and stored at −80 °C for subsequent analysis. Serum liver function levels, including aspartate aminotransferase (AST) and blood fat including glucose, triglyceride, total lipid, total cholesterol, and low-density lipoprotein (LDL) cholesterol were examined at the Green Cross Lab. (Yongin, Republic of Korea). AST, glucose, and LDL cholesterol were analyzed using the Fuji dri-chem slide kit (Fuji Film Co., Tokyo, Japan) with Dri-chem 3500s (Fuji Film Co.), an automatic blood biochemical test analyzer, and triglycerides were measured using a triglycerides reagents kit (Bayer Healthcare LLC, Los Angeles, CA, USA). Total cholesterol was analyzed with a cholesterol reagent kit (Bayer AG Co., Leverkusen, Germany) using ADVIA 1650 biochemistry analyzer (Bayer AG Co.).