Fmoc trp boc oh

Solvents and reagents were purchased from Sigma-Aldrich unless otherwise specified. Triisopropylsilane, piperidine and N,N 0 -diisopropylcarbodiimide (DIC) were of synthesis grade. Rink Amide AM resin (0.71 mmol g À1 ) and Fmoc-Trp(Boc)-OH were purchased from IRIS Biotech GmbH. All other Fmocprotected amino acids and ethyl cyano(hydroxyimino)acetate (Oxyma Pure) were purchased from Novabiochem. Dimethylformamide (DMF) was purchased from J.T. Baker. Dichloromethane (DCM) and acetonitrile (ACN) were purchased from VWR chemical. Solvent exchange was performed in dialysis tubes from Spectrum Laboratories (cellulose ester, MWCO 500-1000 Da, 3.2 cm mL À1 ). Atto550 was purchased from ATTO-TEC GmbH. Atto550-labeled, 22 nucleotide (nt) and 100 nt DNA and their unlabelled complementary stands were purchased from Microsynth. Fetal calf serum (FCS) and phosphate buffer saline (PBS) was purchased from BioConcept. Live cell imaging solution was obtained from Thermo Fisher Scientific. Dulbecco's modified Eagle's medium (DMEM), Opti-MEM, and pen/strep were obtained from Gibco life technologies. CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Promega.

Solvents and reagents were purchased from Sigma-Aldrich unless otherwise specified. Fmoc-Trp(Boc)-OH and Rink Amide AM resin (0.71 mmol g -1 ) were purchased from IRIS Biotech GmbH. Ethyl cyano(hydroxyimino)acetate (Oxyma Pure) and all other Fmoc-protected amino acids were purchased from Novabiochem. Acetonitrile (ACN) and dichloromethane (DCM) were purchased from VWR chemical. Dialysis tubes for solvent exchange were purchased from Spectrum Laboratories (cellulose ester, MWCO 500-1000 Da, 3.2 cm mL -1 ). Atto550-labeled and unlabeled 18 nucleotide (nt) G3139-GAP, 22nt DNA, and scrambled non-specific oligonucleotide were purchased from Microsynth. All oligonucleotide sequences are provided in Fig. 1A. All purified proteins for SPR binding experiments including cysteine-tagged FG domains of human nucleoporins, Nup62, Nup214, human Kapβ1, Kapα, and wild-type Ran (RanWT) were provided by Lim Lab. 41, 42 ACN containing 0.1% TFA with a gradient of 35-50% ACN over 40 min for NLS-peptide, a gradient of 25-60% ACN over 30 min for nonNLS-peptide was used to separate peptides. Peptide purification was monitored at 280 nm. Fractions of purified peptides were collected, lyophilized and stored at -20 °C. The molecular mass of peptides was determined by PerSeptive Biosystems Voyager-DE-PRO time-of-flight mass spectrometer (MALDI-TOF-MS) in positive mode.