Immulite 100

Manufactured by Siemens

Sourced in Germany, United Kingdom

The Immulite 100 is a fully automated immunoassay analyzer designed for in-vitro diagnostic testing. It is capable of performing a wide range of immunoassay tests, including hormone, infectious disease, and allergy tests. The system is equipped with advanced technology to ensure reliable and efficient testing.

Automatically generated - may contain errors

Lab products found in correlation

Human magnetic luminex screening assay, by R&D Systems (1 mentions) Immulite 2000 xpi, by Siemens (1 mentions)

7 protocols using immulite 100

Maternal Hemoglobin and Ferritin Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hemoglobin was measured as part of a standard complete blood count (CBC) by trained research staff in blood from the 2^nd trimester visit, 3^rd trimester visit, in mothers at birth, and in cord blood. Serum was collected by centrifugation and ferritin was measured using an ELISA chemiluminescence assay on an Immulite 100 (Siemens, Munich, Germany). Third trimester ferritin was measured on 586 of 702 the mothers with extra blood available as it was an addition to the standard CBC.

Kupsco A., Estrada-Gutierrez G., Cantoral A., Schnaas L., Pantic I., Amarasiriwardena C., Svensson K., Bellinger D.C., Téllez-Rojo M.M., Baccarelli A.A, & Wright R.O. (2020). Modification of the effects of prenatal manganese exposure on child neurodevelopment by maternal anemia and iron deficiency. Pediatric research, 88(2), 325-333.

+ Open protocol

+ Expand

Chemiluminescent Calcitonin Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Calcitonin in serum was determined by chemiluminescent immunoassays on the automatic Analyzer IMMULITE 100 Siemens (Germany).

Vázquez-Lorente H., Dundjerović D.M., Tatić S.B., Rodríguez-Menéndez S., González-Iglesias H., Gomes C.M., Paunović I.R, & Dragutinović V.V. (2022). Relationship between Trace Elements and Matrix Metalloproteinases 2 and 9 and their Tissue Inhibitors in Medullary Thyroid Carcinoma. Biological Trace Element Research, 201(7), 3225-3232.

+ Open protocol

+ Expand

Multiplex Biomarker Profiling in Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols

The serum levels of sHER2, IL-8, IL-6, DKK-1, and CD44 were simultaneously determined by a custom-made configuration of the Luminex Screening Human Magnetic Assay (R&D Systems). The assays were conducted following the manufacturer’s instructions and were performed on a Luminex 100^TM Qiagen GmbH system (Hilden, Germany). All of the serum samples required a two-fold dilution in calibration diluent. For the analysis, we used a 96-well flat bottom microplate. The measurements for each sample were performed in duplicate, and the average of the two measurements was used. Limits of quantification were determined using the lowest or highest standard point and a percent CV (%CV = 100 × standard deviation/average) of less than 20%. PSA and CRP were determined with Immulite 100 (Siemens Healthcare Diagnostics GmbH, Eschborn, Germany), according to the manufacturer’s instructions.

Theil G., Lindner C., Bialek J, & Fornara P. (2021). Association of Circulating Tumor Cells with Inflammatory and Biomarkers in the Blood of Patients with Metastatic Castration-Resistant Prostate Cancer. Life, 11(7), 664.

+ Open protocol

+ Expand

Serum Biomarker Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the venous blood samples were obtained from the antecubital vein, the samples were centrifuged at 2000 g for 20 min at room temperature for serum separation. Serum samples were then frozen and stored at −20°C for later analysis. The measurements of serum 25(OH)D were performed using electrochemiluminescence immunoassays (ECLIA). Levels of TES and SHBG were measured by electrochemiluminescence immunoassay (Immulite 100; Siemens Healthcare Diagnostics Products Ltd., Gwynedd, United Kingdom). IGF-1 was analyzed by electrochemiluminescence immunoassay (Siemens Immulite 2000 XPI, Siemens Healthcare Diagnostics Products Ltd., Gwynedd, United Kingdom). The inter-assay coefficients of variance (CV) for assays of TES, SHBG, and IGF-I were 7.0%–7.2%, 4.5%–6.2%, and 3.7%–7.4%, and that of sensitivity 0.5 nmol/L, 0.02 nmol/L, and 2.6 nmol/L, respectively.

Laaksi A., Laaksi I., Pihlajamäki H., Vaara J.P., Luukkaala T, & Kyröläinen H. (2023). Associations of serum 25(OH)D levels with physical performance and anabolic hormones in young men. Frontiers in Physiology, 14, 1049503.

+ Open protocol

+ Expand

Maternal Hemoglobin and Ferritin Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Cytokine Concentration Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor necrosis factor alpha (TNF-α), IL-6, IL-10, and IL-8 were measured using a commercially available chemiluminescence kit (IMMULITE 100 Siemens Medical Solutions Diagnostics Limited, Surrey, UK). The sensitivities of the assays were <2 pg/mL for IL-6, <1 pg/mL for IL-10, 2 pg/mL for IL-8, and 4 pg/mL for TNF-α. All samples were processed in duplicate with mean value used if the coefficient of variance was <20%.

Fitrolaki M.D., Dimitriou H., Venihaki M., Katrinaki M., Ilia S, & Briassoulis G. (2016). Increased extracellular heat shock protein 90α in severe sepsis and SIRS associated with multiple organ failure and related to acute inflammatory-metabolic stress response in children. Medicine, 95(35), e4651.

+ Open protocol

+ Expand

Maternal Blood Profiles During Pregnancy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hemoglobin, mean corpuscular volume (MCV), and red blood cell distribution width (RDW) at the 2^nd trimester, 3^rd trimester, and in maternal blood at birth were measured as part of a standard complete blood count (CBC) by trained research staff. A CBC was only performed on a small subset of cord blood samples to estimate neutrophil, lymphocyte, and platelet counts (N=302). Serum was collected by centrifugation, and serum ferritin was measured using an ELISA chemi-luminescence assay on an Immulite 100 (Siemens, Munich, Germany).

Kupsco A., Sanchez-Guerra M., Amarasiriwardena C., Brennan K.J., Estrada-Gutierrez G., Svensson K., Schnaas L., Pantic I., Téllez-Rojo M.M., Baccarelli A.A, & Wright R.O. (2019). Prenatal Manganese and Cord Blood Mitochondrial DNA Copy Number: Effect Modification by Maternal Anemic Status. Environment international, 126, 484-493.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!