Rabbit anti p 4e bp1 thr37 46

Manufactured by Cell Signaling Technology

Rabbit anti-p-4E-BP1 (Thr37/46) is a primary antibody that recognizes the phosphorylated form of the 4E-BP1 (Eukaryotic Translation Initiation Factor 4E-Binding Protein 1) protein at threonine residues 37 and 46.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

4E-BP1 (409 citations) Anti-4E-BP1 (139 citations) P-4E-BP1 (Thr37/46), (43 citations) Rabbit anti-4E-BP1 (38 citations) Rabbit anti-P-4E-BP1 (10 citations) Anti-4E-BP1 (Thr37/46) (2 citations)

4 protocols using rabbit anti p 4e bp1 thr37 46

Automated Histological Evaluation of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histology was performed by HistoWiz Inc. (histowiz.com) using standard operating procedures and fully automated workflow. Samples were processed, embedded in paraffin, and sectioned at 4 μm. Immunohistochemistry was performed on a Bond Rx autostainer (Leica Biosystems) with enzyme treatment (1:1000) using standard protocols. Antibodies used were rabbit anti-p-4E-BP1 (Thr37/46) (Cell Signaling, #2855, 1:800), rabbit anti-p-ERK1/2 (Thr202/Tyr204) (Cell Signaling, #4370, 1:100), and rabbit anti-β-catenin (Cell Signaling #8480, 1:100) antibodies. Bond Polymer Refine anti-rabbit HRP Detection (Leica Biosystems) was used according to manufacturer’s protocol. Sections were then counterstained with hematoxylin, dehydrated and film coverslipped using a TissueTek-Prisma and Coverslipper (Sakura). Whole slide scanning (40x) was performed on an Aperio AT2 (Leica Biosystems). The images were quantified using Halo image analysis software (Indica Labs) using CytoNuclear module.

Kim T., Tafoya E., Chelliah M.P., Lekwuttikarn R., Li J., Sarin K.Y, & Teng J. (2019). Alterations of the MEK/ERK, BMP, and Wnt/β-catenin pathways detected in the blood of individuals with lymphatic malformations. PLoS ONE, 14(4), e0213872.

+ Open protocol

+ Expand

Antibody Selection for Neurodegenerative Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Previously described procedures were followed for these experiments (43 (link), 44 (link)). The antibodies used were rabbit anti-AGAT (orb247515, Biorbyt); mouse anti-GAMT (sc-398936, Santa Cruz Biotechnology); mouse anti–synaptotagmin 1/2 (105011, Synaptic Systems); rabbit anti–PSD-95 (AB9708, Millipore); rabbit anti-Homer1 (ab211415, Abcam); rabbit anti–p-AMPK (Thr172) (2531S, Cell Signaling Technology); rabbit anti–p-UL K (Ser317) (12753, Cell Signaling Technology); rabbit anti-p62 rabbit (8025S, Cell Signaling Technology); rabbit anti–p-mTOR (Ser2448) (2971S, Cell Signaling Technology); rabbit anti-mTOR (2972S, Cell Signaling Technology); rabbit anti–p70 S6 kinase (Thr389) (9205S, Cell Signaling Technology); rabbit anti–p-S6 (Ser240/244) (5364S, Cell Signaling Technology); rabbit anti-S6 (2217S, Cell Signaling Technology); rabbit anti–p-4E-BP1(Thr37/46) (2855S, Cell Signaling Technology); rabbit anti–4E-BP1 (9644S, Cell Signaling Technology); rabbit anti–p-ULK (Ser757) (6888S, Cell Signaling Technology); rabbit anti-GAPDH (5174S, Cell Signaling Technology); rabbit anti-LC3B (ab48394, Abcam); and rabbit anti–cleaved caspase-3 (Asp175) (9661S, Cell Signaling Technology).

Chen H.R., Zhang-Brotzge X., Morozov Y.M., Li Y., Wang S., Zhang H.H., Kuan I.S., Fugate E.M., Mao H., Sun Y.Y., Rakic P., Lindquist D.M., DeGrauw T, & Kuan C.Y. (2021). Creatine transporter deficiency impairs stress adaptation and brain energetics homeostasis. JCI Insight, 6(17), e140173.

+ Open protocol

+ Expand

Antibody Validation and Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: rabbit anti-p70S6K (Cell Signaling Technologies; #2708), rabbit anti-p-p70S6K (Thr389) (Cell Signaling Technologies; #9205), rabbit anti-Akt (Cell Signaling Technologies; #9272), rabbit anti-p-Akt (Thr308) (Cell Signaling Technologies; #13038), rabbit anti-p-Akt (Ser473) (Cell Signaling Technologies; #4051), rabbit anti-ULK1 (Cell Signaling Technologies; #8054), rabbit anti-p-ULK1 (Ser757) (Cell Signaling Technologies; #6888), rabbit anti-TFEB (Cell Signaling Technologies; #4240), rabbit anti-p-TFEB (Ser211) (Cell Signaling Technologies; #37681), rabbit anti-4E-BP1 (Cell Signaling Technologies; #9644), rabbit anti-p-4E-BP1 (Thr37/46) (Cell Signaling Technologies; #2855), rabbit anti-mTOR (Cell Signaling Technologies; #2983), rabbit anti-Raptor (Cell Signaling Technologies; #2280), rabbit anti-HA (Cell Signaling Technologies; #3724), mouse anti-α-tubulin (Thermo; 236-10501), mouse anti-GAPDH (Thermo; AM4300), mouse anti-DYKDDDDK (WAKO; clone 1E6), mouse anti-LAMP1 (Development Studies Hybridoma Bank; H4A3), mouse anti-PIKfyve (Development Studies Hybridoma Bank; 3C9), rabbit anti-TFE3 (Sigma; HPA023881), mouse anti-PP2A-Cα/β (Santa Cruz; sc-80665), and rabbit anti-PPP2CA (Proteintech; 13482-1-AP). Apilimod was from MedChem Express. Okadaic acid was from Santa Cruz. Torin1, YM201636, VPS34-IN1, and FK-506 were from Cayman Chemical.

Hasegawa J., Tokuda E., Yao Y., Sasaki T., Inoki K, & Weisman L.S. (2022). PP2A-dependent TFEB activation is blocked by PIKfyve-induced mTORC1 activity. Molecular Biology of the Cell, 33(3), ar26.

+ Open protocol

+ Expand

Autophagy regulation by pharmacological agents

Check if the same lab product or an alternative is used in the 5 most similar protocols

VES (T3126), chloroquine (C6628), 3-methyladenine (M9281) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (88417) were purchased from Sigma. Primary antibodies used for western blotting were rabbit anti-LC3 (Sigma, L7543), rabbit anti-Akt (Cell Signaling, 9272), rabbit anti-p-Akt (S473) (Cell Signaling, 9271S), rabbit anti-mTOR (Cell Signaling, 2972S), rabbit anti-p-mTOR (Ser2448) (Cell Signaling,5536S), rabbit anti-p70S6K (Cell Signaling,5707S), rabbit anti-p-p70S6K (T389) (Cell Signaling, 9205S), rabbit anti-4E-BP-1(Cell Signaling, 8594S), rabbit anti-p-4E-BP-1 (Thr37/46) (Cell Signaling, 9459S), rabbit antip38 MAPK (Cell Signaling,,8690S), rabbit anti-p-p38 MAPK (The180/Tyr1820) (Cell Signaling, 4092), rabbit anti-5'Adenosine monophosphate-activated protein kinase α (AMPKα) (Cell Signaling, 5831S), rabbit anti-p-AMPKα (Thr172) (Cell Signaling, 2535S) and rabbit anti-Actin (Santa Cruz Biotechnology, sc-1616). Alkaline phosphatase conjugated second antibody used for western blotting was anti-rabbit IgG (promega, S373B). Green fluorescent protein—microtubule associated protein 1 light chain 3 (GFP-LC3) plasmid was obtained from YRGene, China (Yrbio, VXY0542). All cell culture solutions were obtained from Thermo and all plastic-ware was obtained from Nunc, unless otherwise stated.

Hou L., Li Y., Song H., Zhang Z., Sun Y., Zhang X, & Wu K. (2015). Protective Macroautophagy Is Involved in Vitamin E Succinate Effects on Human Gastric Carcinoma Cell Line SGC-7901 by Inhibiting mTOR Axis Phosphorylation. PLoS ONE, 10(7), e0132829.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!