Facs calibur

Manufactured by Roche

The FACS-Calibur is a flow cytometry instrument designed for cell analysis and sorting. It utilizes laser technology to detect and measure various physical and fluorescent characteristics of cells or other particles passing through a fluid stream. The FACS-Calibur is capable of analyzing multiple parameters simultaneously, providing researchers and clinicians with valuable data for a wide range of applications.

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Lab products found in correlation

Annexin 5 fitc, by Roche (1 mentions) Propidium iodide, by Merck Group (1 mentions) Rnase a, by Roche (1 mentions)

2 protocols using facs calibur

Quantifying Apoptosis via Annexin V Flow

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KC or KPC cells were treated as indicated, trypsinized, washed in H/S, stained for 15 min with FITC-Annexin V (Roche), and analyzed by flow cytometry on a FACS-Calibur. Controls were permeabilized for 5 min with 0.1% Triton X-100 at room temperature before incubation with FITC-Annexin V.
Cell death was confirmed by Trypan Blue staining and counting of dead cells.

Patel S.H., Bachmann M., Kadow S., Wilson G.C., Abdel-Salam M.M., Xu K., Keitsch S., Soddemann M., Wilker B., Becker K.A., Carpinteiro A., Ahmad S.A., Szabo I, & Gulbins E. (2023). Simultaneous targeting of mitochondrial Kv1.3 and lysosomal acid sphingomyelinase amplifies killing of pancreatic ductal adenocarcinoma cells in vitro and in vivo. Journal of Molecular Medicine (Berlin, Germany), 101(3), 295-310.

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Cell Cycle Analysis by Flow Cytometry

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Cells were treated with either TRC-794 or DMSO for 24 hours and subsequently fixed in 100% ethanol at −20°C overnight. Cells were then resuspended in propidium iodide (Sigma-Aldrich) and RNAseA (Roche) and allowed to stain for 20 minutes prior to flow cytometry analysis, performed on the FACSCalibur.

Wiredja D.D., Ayati M., Mazhar S., Sangodkar J., Maxwell S., Schlatzer D., Narla G., Koyutürk M, & Chance M.R. (2017). Phosphoproteomics Profiling of Non-Small Cell Lung Cancer Cells Treated with a Novel Phosphatase Activator. Proteomics, 17(22), 10.1002/pmic.201700214.

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