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Rx821002

Manufactured by Bio-Techne
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Sourced in United Kingdom

RX821002 is a laboratory equipment product manufactured by Bio-Techne. It is a device designed for laboratory use, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

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Lab products found in correlation

Yohimbine hydrochloride, by Bio-Techne (2 mentions) Clonidine hydrochloride, by Bio-Techne (2 mentions) Wb4101, by Bio-Techne (2 mentions) Vasopressin, by Bio-Techne (2 mentions) L phenyalanine, by Merck Group (2 mentions) Sb206553, by Bio-Techne (2 mentions) Gr127935, by Bio-Techne (2 mentions) Nsd1015, by Merck Group (2 mentions) Quinpirole hydrochloride, by Bio-Techne (1 mentions) 3h sch23390, by PerkinElmer (1 mentions) Pertussis toxin, by Merck Group (1 mentions) Ro 105824 dihydrochloride, by Bio-Techne (1 mentions) 3h ym 09151 2, by PerkinElmer (1 mentions) L norepinephrine bitartrate salt monohydrate, by Merck Group (1 mentions) Dopamine hydrochloride, by Merck Group (1 mentions) Flunixin meglumine, by Merck & Co (1 mentions) Tribromoethanol, by Merck Group (1 mentions) Urethane, by Merck Group (1 mentions) Propranolol, by Merck Group (1 mentions) Prazosin hydrochloride, by Bio-Techne (1 mentions)

5 protocols using rx821002

1

Radioligand Binding Assay Protocol

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Dopamine hydrochloride and L-(-)-norepinephrine (+)-bitartrate salt monohydrate were purchased from Sigma. (-) quinpirole hydrochloride, clonidine hydrochloride, 7-OH-PIPAT maleate, RO-105824 dihydrochloride, RX821002 and yohimbine hydrochloride were purchased from Tocris. [3H]RX821002 (63.9 Ci/mmol), [3H]SCH 23390 (81.9 Ci/mmol) and [3H]YM-09151-2 (84.4 Ci/mmol) were from Perkin-Elmer. Pertussis toxin was purchased from Sigma.
Sánchez-Soto M., Casadó-Anguera V., Yano H., Bender B.J., Cai N.S., Moreno E., Canela E.I., Cortés A., Meiler J., Casadó V, & Ferré S. (2018). α2A- and α2C-Adrenoceptors as Potential Targets for Dopamine and Dopamine Receptor Ligands. Molecular neurobiology, 55(11), 8438-8454.
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2

Adrenoceptor Antagonist Cocktail Protocol

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WB4101 (selective α1-adrenoceptor antagonist; Tocris, Westwoods Business Park, Ellisville, MO, USA; cat. # 0946), RX821002 (selective α2-adrenoceptor antagonist; Tocris, cat. # 1324), propranolol (β-adrenoceptor antagonist; Sigma–Aldrich, St. Louis, Missouri, USA; cat. # P0884), tribromoethanol (Sigma–Aldrich, cat. # T48402), and urethane (Sigma–Aldrich; cat. # U2500) were dissolved in saline (NaCl 0.9%). Flunixin meglumine (Banamine, Schering Plough, Cotia, São Paulo, Brazil) and the polyantibiotic preparation of streptomycins and penicillins (Pentabiotico, Fort Dodge, Campinas, São Paulo, Brazil) were used as provided.
Oliveira L.A., Pollo T.R., Rosa E.A., Duarte J.O., Xavier C.H, & Crestani C.C. (2021). Both Prelimbic and Infralimbic Noradrenergic Neurotransmissions Modulate Cardiovascular Responses to Restraint Stress in Rats. Frontiers in Physiology, 12, 700540.
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3

Detailed ACSF compositions for in vitro experiments

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Two kinds of ACSF were used in in vitro experiments: mACSF in the dissection dish before recording and nACSF in the recording chamber. mACSF was composed of (in mM) 118 NaCl, 24 NaHCO3, 1.5 CaCl2, 3 KCl, 5 MgCl2, 1.4 NaH2PO4, 1.3 MgSO4, 25 d-glucose, and 1 kynurenic acid. nACSF was composed of (in mM) 122 NaCl, 25 NaHCO3, 2.5 CaCl2, 3 KCl, 1 MgCl2, 0.5 NaH2PO4, and 12 d-glucose. Both types of ACSF were saturated with carbogen (95% O2-5% CO2) and maintained at pH 7.4. The drugs added to the nACSF were 5-HTP, clorgyline, pargyline, tryptamine, L-tryptophan, tyramine, L-tyrosine, 2-phenylethylamine, L-phenyalanine, NSD1015 (Sigma-Aldrich), α-methyl-5-HT, octopamine, SB206553, RX821002, GR127935, dobutamine, vasopressin (Tocris) and zolmitriptan (AstraZenica). All drugs were first dissolved as a 10 – 50 mM stock solution in water before final dilution in ACSF for in vitro ventral root reflexes recording and DIC microscopy or in saline for in vivo oxygen measurements and two photon microscopy, with the exception of zolmitriptan, which was dissolved in minimal amounts of DMSO (DMSO final concentration in nACSF was 0.04%). DMSO alone had no effect on in vitro LLR in vehicle controls, as compare to nACSF control state.
Li Y., Lucas-Osma A.M., Black S., Bandet M.V., Stephens M.J., Vavrek R., Sanelli L., Fenrich K.K., Di Narzo A.F., Dracheva S., Winship I.R., Fouad K, & Bennett D.J. (2017). Pericytes impair capillary blood flow and motor function after chronic spinal cord injury. Nature medicine, 23(6), 733-741.
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4

Detailed ACSF compositions for in vitro experiments

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Two kinds of ACSF were used in in vitro experiments: mACSF in the dissection dish before recording and nACSF in the recording chamber. mACSF was composed of (in mM) 118 NaCl, 24 NaHCO3, 1.5 CaCl2, 3 KCl, 5 MgCl2, 1.4 NaH2PO4, 1.3 MgSO4, 25 d-glucose, and 1 kynurenic acid. nACSF was composed of (in mM) 122 NaCl, 25 NaHCO3, 2.5 CaCl2, 3 KCl, 1 MgCl2, 0.5 NaH2PO4, and 12 d-glucose. Both types of ACSF were saturated with carbogen (95% O2-5% CO2) and maintained at pH 7.4. The drugs added to the nACSF were 5-HTP, clorgyline, pargyline, tryptamine, L-tryptophan, tyramine, L-tyrosine, 2-phenylethylamine, L-phenyalanine, NSD1015 (Sigma-Aldrich), α-methyl-5-HT, octopamine, SB206553, RX821002, GR127935, dobutamine, vasopressin (Tocris) and zolmitriptan (AstraZenica). All drugs were first dissolved as a 10 – 50 mM stock solution in water before final dilution in ACSF for in vitro ventral root reflexes recording and DIC microscopy or in saline for in vivo oxygen measurements and two photon microscopy, with the exception of zolmitriptan, which was dissolved in minimal amounts of DMSO (DMSO final concentration in nACSF was 0.04%). DMSO alone had no effect on in vitro LLR in vehicle controls, as compare to nACSF control state.
Li Y., Lucas-Osma A.M., Black S., Bandet M.V., Stephens M.J., Vavrek R., Sanelli L., Fenrich K.K., Di Narzo A.F., Dracheva S., Winship I.R., Fouad K, & Bennett D.J. (2017). Pericytes impair capillary blood flow and motor function after chronic spinal cord injury. Nature medicine, 23(6), 733-741.
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5

Pharmacological Agents for Neuroscience Research

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ARC-239, BRL-44408, clonidine hydrochloride, dexmedetomidine hydrochloride, efaroxan hydrochloride, guanabenz acetate, guanfacine hydrochloride, idazoxan hydrochloride, JP-1302, prazosin hydrochloride, rauwolscine hydrochloride, RS-79948, RX-821002, terazosin, UK-14304, WB-4101 and yohimbine hydrochloride were acquired from Tocris (Ellisville, MO). Atipamezole was from Orion Corporation (Espoo, Finland) and isoflurane was from Abbott Diagnostics (Chicago, IL). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
Biggane J.P., Xu K., Goldenstein B.L., Davis K.L., Luger E.J., Davis B.A., Jurgens C.W., Perez D.M., Porter J.E, & Doze V.A. (2022). Pharmacological characterization of the α2A-adrenergic receptor inhibiting rat hippocampal CA3 epileptiform activity: Comparison of ligand efficacy and potency. Journal of receptor and signal transduction research, 42(6), 580-587.
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