The lung tissue samples of the rabbits were collected into sterile tubes to isolate bacterial strains. A lung tissue portion of 3 × 3 mm was taken aseptically and added into 10 mL Brain Heart Infusion Broth (BHI, CM1135, Oxoid). The bacteria were grown at 37 °C by shaking at 120 rpm for 24 h. Then, the grown bacteria were inoculated onto Mannitol Salt Agar (MSA, CM085, Oxoid) and incubated at 37 °C for 48 h to isolate S. aureus strains. Presumptive S. aureus strains were selected from MSA and inoculated into Tryptone Soy Broth (TSB, CM129, Oxoid). Then, 20% (v/v) glycerol stock solutions were prepared and stored at −80 °C for further analysis. DNA extraction from presumptive S. aureus strains was carried out as described by Sudagidan et al. [35 ]. S. aureus strains were identified by PCR using specific primers for nuc [36 ], coa [37 (link)], and spa genes [38 (link)]. The presence of the PVL gene in the isolated strains was searched by PCR using lukSF-PV-specific primers [39 (link)].
Cm085
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The CM085 is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 4,000 rpm and a maximum RCF of 2,000 x g. The centrifuge accommodates a variety of rotor options to support different sample volumes and tube sizes. Its compact footprint and user-friendly interface make it suitable for use in various laboratory settings.
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Lab products found in correlation
2 protocols using cm085
1
Isolation and Identification of S. aureus from Rabbit Lung Tissues
2
Quantifying Staphylococcus aureus in Rinse Fluids
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Using spread plate method, 0.5 ml of each 4 consecutive 10 fold serial dilutions (10 -1 to 10 -4 ) of the rinse fluid were inoculated in triplicates into mannitol salt agar (Oxoid CM 085, UK). The plates were incubated at 37°C for 24 h and the number of yellow colonies recorded per dilution. CFU/ml, CFU/cm 2 and the respective logs were then calculated. For confirmation of coagulase positive S. aureus, four suspect colonies per dilution were sub-cultured onto blood agar (OXOID CM0271, UK) and incubated at 37°C for 24 h. Golden yellow and cream white colonies were tested for catalase and coagulase activity.
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