Anti cd4 monoclonal antibody clone gk1.5

Manufactured by BioXCell

The Anti-CD4+ monoclonal antibody (clone GK1.5) is a laboratory reagent used for research purposes. This antibody specifically binds to the CD4 surface antigen expressed on T helper cells. It can be used in various immunological techniques to identify and study CD4+ T cells.

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Lab products found in correlation

Rat igg2b isotype control antibody, by BioXCell (2 mentions) Clone ltf 2, by BioXCell (1 mentions) Fluorochrome conjugated anti mouse monoclonal antibodies, by BioLegend (1 mentions) Igg2b isotype control, by BioXCell (1 mentions)

Spelling variants of this product

Anti-CD4 (clone GK1.5, (90 citations) Anti-CD4 (GK1.5, (39 citations) Anti-CD4 (32 citations) Clone GK1.5 (27 citations) Anti-CD4 antibody (clone GK1.5, (20 citations) CD4 (clone GK1.5, (8 citations) Anti-CD4 antibody (GK1.5, (7 citations) GK1.5 antibody (6 citations) CD4 (GK1.5) (3 citations) GK1.5 anti-CD4 (2 citations)

5 protocols using anti cd4 monoclonal antibody clone gk1.5

CD4+ T Cell Depletion in Mice

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For CD4⁺ T cell depletion experiments, mice are treated with 0.1 mg of anti-CD4 monoclonal antibody (clone GK1.5; Bio X Cell, West Lebanon, New Hampshire) or 0.1 mg of isotype-matched control antibody (clone LTF-2; Bio X Cell) via intraperitoneal injections on the days − 1 and + 3 relative to the start of DSS administration [38 ]. Depletion of CD4⁺ T cell was confirmed by FCM analysis of T cell population in the peripheral blood and spleen by staining with fluorochrome-conjugated anti-mouse monoclonal antibodies (BioLegend) against CD3 (clone 17A2) and CD4 (clone RM4.4), CD8 (clone 53-6.7) and CD11b (clone M1/70).

Kojima F., Sekiya H., Hioki Y., Kashiwagi H., Kubo M., Nakamura M., Maehana S., Imamichi Y., Yuhki K.I., Ushikubi F., Kitasato H, & Ichikawa T. (2022). Facilitation of colonic T cell immune responses is associated with an exacerbation of dextran sodium sulfate–induced colitis in mice lacking microsomal prostaglandin E synthase-1. Inflammation and Regeneration, 42, 1.

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CD4+ Depletion Prior to Pneumonia

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For CD4⁺ depletion studies Het Imm (i.t.) mice were administered an anti-CD4⁺ monoclonal antibody (clone GK1.5) or a rat IgG2b isotype control antibody (BioXcell, West Lebanon, NH). Antibodies were diluted in sterile saline and delivered at doses of 500 μg i.p. and 100μg i.n. (under anesthesia) both 72 and 24 hours prior to the i.t. Sp3 pneumonia challenge.

Smith N.M., Wasserman G.A., Coleman F.T., Hilliard K.L., Yamamoto K., Lipsitz E., Malley R., Dooms H., Jones M.R., Quinton L.J, & Mizgerd J.P. (2017). Regionally Compartmentalized Resident Memory T Cells Mediate Naturally Acquired Protection Against Pneumococcal Pneumonia. Mucosal immunology, 11(1), 220-235.

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Pneumococcal Infection in Naive Mice

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Naive mice being ushered through pneumococcal experience were administered anti-CD4 monoclonal antibody (clone GK1.5) or IgG2b isotype control (BioXcell) via i.p. instillations (500 μg/100 μL) and i.n. instillations (100 μg/100 μL). The antibodies were delivered 1 day before and then both 1 day and 3 days after each of the 2 infections with Sp19F.

Arafa E.I., Shenoy A.T., Barker K.A., Etesami N.S., Martin I.M., Lyon De Ana C., Na E., Odom C.V., Goltry W.N., Korkmaz F.T., Wooten A.K., Belkina A.C., Guillon A., Forsberg E.C., Jones M.R., Quinton L.J, & Mizgerd J.P. (2022). Recruitment and training of alveolar macrophages after pneumococcal pneumonia. JCI Insight, 7(5), e150239.

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CD4+ Depletion Prior to Pneumonia

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CD4+ T Cell Depletion in Mice

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To deplete CD4⁺ T cells, mice were first injected i.p. with 200ug of anti-CD4 monoclonal antibody (clone GK1.5, Bio X Cell) or isotype control in 1X PBS, and then injected with 100ug i.p. every 3–4 days afterwards for a total of 1 month of treatment.

Lobby J.L., Uddbäck I., Scharer C.D., Mi T., Boss J.M., Thomsen A.R., Christensen J.P, & Kohlmeier J.E. (2022). Persistent antigen harbored by alveolar macrophages enhances the maintenance of lung-resident memory CD8+ T cells. Journal of immunology (Baltimore, Md. : 1950), 209(9), 1778-1787.

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