Penicillin streptomycin

Manufactured by Sino Biological

Sourced in United States, China

Penicillin-streptomycin is a combination of two antibiotics, penicillin and streptomycin, commonly used in cell culture media to prevent bacterial contamination. It inhibits the growth and reproduction of a wide range of gram-positive and gram-negative bacteria.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Hek293 cell complete medium, by Sino Biological (1 mentions) Erythropoietin, by Sino Biological (1 mentions) Kit ligand, by R&D Systems (1 mentions) Macrophage colony stimulating factor, by Sino Biological (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Grace s insect cell culture medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Sino Biological (1 mentions) Sim sf expression medium, by Sino Biological (1 mentions) Epidermal growth factor (egf), by Sino Biological (1 mentions)

Close spelling variants of this product

Streptomycin (3 citations) Penicillin (3 citations)

5 protocols using penicillin streptomycin

SARS-CoV-2 Virus Propagation and Characterization

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Vero E6 cells were cultured in Dulbecco's modified Eagle medium (DMEM) containing sodium pyruvate, 2 mM l-glutamine, 1% penicillin-streptomycin (Gibco) and 10% Fetal Bovine Serum (Gibco). Adherent Spodoptera frugiperda 9 (Sf9) cells from Life Technologies (USA) were cultured in Grace's Insect Cell Culture medium (Gibco) with 10% Fetal Bovine Serum and 1% penicillin-streptomycin at 27 °C; Sf9 suspension cells were cultured in SIM SF Expression Medium (Sino Biological) with 1% penicillin-streptomycin at 27 °C. Lactobacillus MG1363 was cultured in M17 medium (Cat: HB0391, Qingdao Hope Biotechnology Company) with 1% glucose at 30 °C and was stored in our laboratory.
The isolated SARS-CoV-2 virus (BetaCoV/Beijing/IME-BJ05-2020, abbreviated as BJ05, GenBank accession No. MT291835) and a SARS-CoV-2 mouse-adapted strain (C57MA14) were grown in Vero E6 cells for 3 days at 37 °C and 5% CO₂. The C57MA14 strain was passaged 14 times in aged C57BL/6 N mice. Furthermore, BJ05 and C57MA14 were cultured as described previously (Yan et al., 2022 ).

Su R., Shi Z., Li E., Zhu M., Li D., Liu X., Sun Y., Feng N., Wang J., Wang T., Xia X., Sun W, & Gao Y. (2023). A Trim-RBD-GEM vaccine candidate protects mice from SARS-CoV-2. Virology, 585, 145-154.

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Purification of Vaccinia Virus Tiantan Strain

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Vaccinia virus (VACV) Tiantan strain (VTT) was grown in Vero cells by using a multiplicity of infection of 0.01 and harvested at 48 h post-infection. Cells were lysed by 3 freeze/thaw cycles. The plaque-forming unit (PFU) of viral stocks was titrated on Vero cells by a plaque-forming assay using crystal violet staining. The virus was inactivated with 0.025% β-propiolactone (v/v) before purification by ultracentrifugation through a 36% sucrose cushion.^{34 (link)} African green monkey kidney (Vero) cells were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Shanghai VivaCell Biosciences Ltd, Shanghai, China) and 1% penicillin/streptomycin (Thermo Fisher Scientific) at 37 °C with 5% CO₂. Human embryonic kidney 293F (HEK293F) cells were cultured in HEK293 Cell Complete Medium (Sino Biological Inc., Beijing, China) supplemented with 1% penicillin/streptomycin.

Li E., Guo X., Hong D., Gong Q., Xie W., Li T., Wang J., Chuai X, & Chiu S. (2023). Duration of humoral immunity from smallpox vaccination and its cross-reaction with Mpox virus. Signal Transduction and Targeted Therapy, 8, 350.

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Cell Culture and Differentiation Protocols

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HEK293T and HeLa cells were purchased from the American Type Culture Collection (ATCC) and propagated in Dulbecco modified Eagle medium with 4.5 g/L glucose, 10% fetal bovine serum, and 2 mM glutamine at 37°C, 5% CO₂ in a humidified incubator. G1E-ER4 cells were maintained in Iscove modified Dulbecco medium with 15% fetal bovine serum, 100 U/mL penicillin-streptomycin, 2 U/mL erythropoietin (Sino Biological Inc.), monothioglycerol (1:10,000), and 50 ng/mL Kit-ligand (R&D Systems). GATA1-mediated differentiation was induced by the addition of 100 nmol of β-estradiol to a cell culture at a density of 2×10⁵ cells/mL. All cell lines were tested for mycoplasma.

Maio N., Kim K.S., Holmes-Hampton G., Singh A, & Rouault T.A. (2019). Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica, 104(9), 1756-1767.

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Isolation and Differentiation of Bone Marrow-Derived Macrophages

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BMDMs were isolated from Fam76b^-/- and WT C57BL/6 mice by flushing the femur with Dulbecco’s modified Eagle medium (DMEM) supplemented with 3% fetal bovine serum (FBS). Cells were plated for 4 hr to allow the non-monocytes to adhere to the surface of culture dish. Then, the monocytes in the culture supernatant were collected and centrifuged at 250×g and then seeded in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, 1% L-glutamate, and 10 ng/mL macrophage colony-stimulating factor (Sino Biological Inc, Beijing, China). The fresh medium containing 10 ng/mL macrophage colony-stimulating factor was changed every 2 days to induce differentiation of the cells into macrophages. After 6 days in culture, BMDMs were collected for the following experiments.

Wang D., Zheng X., Chai L., Zhao J., Zhu J., Li Y., Yang P., Mao Q, & Xia H. (2023). FAM76B regulates NF-κB-mediated inflammatory pathway by influencing the translocation of hnRNPA2B1. eLife, 12, e85659.

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Pancreatic Cancer Cell Lines Cultivation

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Human PDAC cell lines (Mia PaCa-2 and SUIT-2) and a nonmalignant pancreatic epithelial (hTERT-HPNE) cell line were obtained from Drs. Wun-Shaing Wayne Chang and Li-Tzong Chen, National Institute of Cancer Research (National Health Research Institutes, Miaoli, Taiwan). Mia PaCa-2 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Corning), 2.5% horse serum, and 1% penicillin-streptomycin (TOKU-E, Bellingham, WA, USA). SUIT-2 cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 1% penicillin-streptomycin. hTERT-HPNE cells were cultured in low-glucose DMEM (Hyclone, Thermo Scientific) supplemented with 5% FBS, 1% penicillin-streptomycin, and 10 ng/mL epidermal growth factor (Sino Biological, Wayne, PA, USA). These cells were free of mycoplasma contamination, and their identities were confirmed by short tandem repeat (STR) profiling at the BCRC and Center for Genomic Medicine, National Cheng Kung University (NCKU; Tainan, Taiwan). All cells were maintained at 37 °C in a 5% CO₂ atmosphere. For drug treatment, cells were pre-cultured to 60–70% confluence and supplemented with medium containing BA (Sigma-Aldrich, St. Louis, MO, USA) for 24 or 48 h at indicated doses.

Chiu C.F., Chang H.Y., Huang C.Y., Mau C.Z., Kuo T.T., Lee H.C, & Huang S.Y. (2021). Betulinic Acid Affects the Energy-Related Proteomic Profiling in Pancreatic Ductal Adenocarcinoma Cells. Molecules, 26(9), 2482.

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