Mouse monoclonal antibody 1a4

Manufactured by Merck Group

Mouse monoclonal antibody (1A4) is a laboratory reagent used in various research and diagnostic applications. It is a purified antibody produced by a mouse hybridoma cell line. The antibody specifically recognizes and binds to a target antigen, making it a useful tool for identifying and studying the target molecule.

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Lab products found in correlation

Analysis 3, by Olympus (1 mentions) Tcs sp8 confocal microscope, by Leica (1 mentions)

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Monoclonal mouse antibody (7 citations) Monoclonal mouse (2 citations) Anti-α-smooth muscle actin mouse monoclonal antibody (clone 1A4) (2 citations)

2 protocols using mouse monoclonal antibody 1a4

Liver Collagen and αSMA Quantification

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Liver collagen content was assessed by Sirius red staining and scored by imaging analysis (AnalySIS 3.1, Soft Imaging System) as described [14 (link)]. αSMA was stained with a mouse monoclonal antibody (1A4, Sigma) diluted 1:400.

Sobrevals L., Enguita M., Quiroga J., Prieto J, & Fortes P. (2016). Insulin-Like Growth Factor I (IGF-I) Expressed from an AAV1 Vector Leads to a Complete Reversion of Liver Cirrhosis in Rats. PLoS ONE, 11(9), e0162955.

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Indirect Immunofluorescence Staining for αSMA

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Indirect immunofluorescence staining was performed as previously described (Coric et al., 2004 (link)). Expression of smooth muscle actin (αSMA) was detected using mouse monoclonal antibody 1A4 (Sigma Aldrich) at 1:400 dilution. After staining cell were mounted in Mowiol medium containing 1 μg/ml DAPI to counterstain cell nuclei medium and examined under a Leica TCS SP8 confocal microscope.

Cazaña-Pérez V., Cidad P., Donate-Correa J., Martín-Núñez E., López-López J.R., Pérez-García M.T., Giraldez T., Navarro-González J.F, & Alvarez de la Rosa D. (2018). Phenotypic Modulation of Cultured Primary Human Aortic Vascular Smooth Muscle Cells by Uremic Serum. Frontiers in Physiology, 9, 89.

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