Takara minibest whole blood genomic dna extraction kit

Manufactured by Takara Bio

Sourced in China, Japan

The TaKaRa MiniBEST Whole Blood Genomic DNA Extraction Kit is designed for the rapid and efficient extraction of genomic DNA from whole blood samples. The kit utilizes a simple and effective protocol to isolate high-quality DNA suitable for downstream applications such as PCR, sequencing, and other molecular biology techniques.

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DNA extraction kit (50 citations) Takara kit (48 citations) DNA blood kit (4 citations) Blood Genomic DNA Extraction kit (2 citations)

5 protocols using takara minibest whole blood genomic dna extraction kit

Inflammatory Genetic Variant Screening

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Fourth-seven inflammation genes were selected based on a literature review. For each candidate gene, SNPs associated with human disease susceptibility, prognosis, and treatment outcomes were searched in PubMed. For the coding SNPs of candidate genes not reported in the literature, those with allele frequency greater than 0.05 in Chinese population were selected. Furthermore, only SNPs with a minimum allele frequency (MAF) greater than 0.05 were selected for genotyping in order to improve the statistical efficiency. Finally, a total of 47 SNPs were selected from 47 inflammation genes (Table S1). Genomic DNA was extracted from whole blood using the TaKaRa MiniBEST Whole Blood Genomic DNA Extraction Kit (TakaRa, Dalian, China) according to the manufacturer’s instructions. All SNPs were genotyped using a PCR-ligation detection reaction (LDR) method as previously described (3 (link)).

Zhang X., Ye T., Li M., Yan H., Lin H., Lu H., Qi Z., Sheng H, & He C. (2022). Association of Polymorphisms in Inflammation Genes With the Prognosis of Advanced Non-Small Cell Lung Cancer Patients Receiving Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors. Frontiers in Oncology, 12, 836117.

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Baculovirus Expression and Drug Metabolism

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TaKaRa MiniBEST Whole Blood Genomic DNA Extraction Kit, PrimeSTAR HS DNA polymerase, restriction enzymes, and T4 DNA ligase were purchased from Takara Bio Inc. (Kusatsu, Japan). Spodoptera frugiperda (Sf) 21 cells, fetal bovine serum, Sf-900™ III SFM insect culture medium, Cellfectin II Reagent, and the Bac-to-Bac™ Baculovirus Expression System were purchased from Invitrogen (Carlsbad, CA, United States). Tolbutamide, 4-hydroxy Tolbutamide, losartan, losartan carboxylic acid, warfarin, 7-hydroxy warfarin, diazepam and midazolam (internal standard [IS]) were purchased from Toronto Research Chemicals (Toronto, Canada). Rabbit polyclonal anti-CYP2C9 antibody and mouse monoclonal anti-OR antibody were obtained from Abcam (Cambridge, United Kingdom) and Santa Cruz Biotechnology (Santa Cruz, CA, United States), respectively. A SuperSignal West Pico Trial Kit was obtained from Thermo Scientific (Rockford, IL, United States). A regeneration system for the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Promega (Madison, WI, United States). High-performance liquid chromatography-grade organic solvents and liquid chromatography–mass spectrometry-grade acetonitrile were purchased from Merck (Darmstadt, Germany). All other chemicals and reagents used were of the highest purity available.

Zhou X.Y., Lu X.R., Li Y.H., Ma Y.Q., Zhao S.W., Wang F., Xu R.A., Hu G.X, & Cai J.P. (2021). Identification and Enzymatic Activity Evaluation of a Novel CYP2C9 Allelic Variant Discovered in a Patient. Frontiers in Pharmacology, 12, 619339.

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DNA and RNA Extraction from Blood and Skin

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Genomic DNA was extracted from the blood samples using a TaKaRa MiniBEST Whole Blood
Genomic DNA Extraction Kit (TaKaRa, Dalian, China). Total RNA was extracted from the back
skin using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA; Cat no. 15596026)
in accordance with the manufacturer’s recommendations. The integrity and yield of the
genomic DNA and total RNA were assessed and verified using agarose gel electrophoresis and
a NanoDrop spectrophotometer (Thermo Fisher Scientific), respectively. Stock DNA samples
at a concentration of approximately 25 ng/μL were stored at −20 °C for Kompetitive
Allele-Specific PCR (KASP) analysis.

Zhang X., Qi Y., Pang Y., Yuan B, & Li X. (2023). Identification of Polymorphisms in the HERC2-OCA2 Gene Locus and their Association with Feather Color in Quail. The Journal of Poultry Science, 60(2), 2023013.

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CARD15/NOD2 Gene Extraction Protocol

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All blood samples were taken as part of routine care and venous blood with anticoagulant was stored at -80˚C until DNA extraction. Genomic DNA was extracted with TaKaRa MiniBEST Whole Blood Genomic DNA Extraction Kit (TaKaRa Bio, Japan) and DNA samples were stored at -20˚C. The CARD15/NOD2 gene (Gene ID: 444867), located on chromosome 18 (AC_000175.1) was used to design 21 primer pairs using Oligo Primer Analysis software v.5 (Molecular Biology Insights, Inc., USA). All primers were synthesized by the Beijing Genomics Institute (BGI), China (S1 Table).

Wang Y., Wang S., Liu T., Tu W., Li W., Dong G., Xu C., Qin B., Liu K., Yang J., Chai J., Shi X, & Zhang Y. (2015). CARD15 Gene Polymorphisms Are Associated with Tuberculosis Susceptibility in Chinese Holstein Cows. PLoS ONE, 10(8), e0135085.

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SARS-CoV-2 and HPV Detection Protocols

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Clinical oropharyngeal swab samples (Table S11) from patients infected with SARS-CoV-2 were collected and tested by the Centers for Disease Control and Prevention (CDC) Wuhan, China. The viral RNA extraction was performed using a KingFisher Flex nucleic acid extraction system (ThermoFisher, USA). Clinical cervical swab samples (Table S11) from patients infected with different HPV subtypes were collected by the Medical College of Hubei University of Arts and Sciences. The genomic DNA was extracted using Takara MiniBEST Whole Blood Genomic DNA Extraction Kit (Takara Biomedical Technology). The extracted nucleic acids were amplified with the Blood Directed PCR Kit V2 to confirm the subtypes of HPV.

Wang L., He R., Lv B., Yu X., Liu Y., Yang J., Li W., Wang Y., Zhang H., Yan G., Mao W., Liu L., Wang F, & Ma L. (2021). Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV. Talanta, 227, 122154.

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