Lc 20a system

Manufactured by Shimadzu

Sourced in Japan, United States

The Shimadzu LC-20A system is a high-performance liquid chromatography (HPLC) instrument designed for analytical applications. It provides reliable separation, detection, and quantification of chemical compounds in various sample types. The LC-20A system features a modular design, allowing for flexibility in configuration and customization to meet specific analytical requirements.

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Lab products found in correlation

Sil 20a, by Shimadzu (6 mentions) Lc 20ab, by Shimadzu (4 mentions) Spd m20a, by Shimadzu (4 mentions) Zorbax sb c18 column, by Agilent Technologies (2 mentions) Symmetry c18 column, by Waters Corporation (2 mentions) Shim pack xr ods column, by Shimadzu (2 mentions) Amazon sl mass spectrometer, by Bruker (2 mentions) Lc 20ad, by Shimadzu (2 mentions) Lcms 2010ev, by Shimadzu (2 mentions) Microflex lrf, by Bruker (1 mentions) Lc 20adxr, by Shimadzu (1 mentions) Sil 20a xr, by Shimadzu (1 mentions) Poroshell 120 ec c18 column, by Agilent Technologies (1 mentions) Mestrenova 12, by Mestrelab Research (1 mentions) Topspin software, by Bruker (1 mentions) Choline sulfate, by Cambridge Isotopes (1 mentions) Monopotassium phosphate kh2po4, by Merck Group (1 mentions) Sodium deuteroxide solution, by Merck Group (1 mentions) Luna 5 micron c18 column, by Phenomenex (1 mentions) Symmetry c8 column, by Waters Corporation (1 mentions)

Spelling variants of this product

LC-20A (357 citations) LC-20A HPLC system (91 citations) LC-20A Prominence HPLC system (15 citations) Prominence LC-20A series HPLC system (13 citations) LC-20A series HPLC system (4 citations) LC-20A UHPLC system (3 citations) LC 20A RP-HPLC system (2 citations) Prominence LC-20A series system (2 citations) Prominence LC-20A UFLC Stack HPLC system (2 citations) LC-20A UFLC system (1 citations)

67 protocols using lc 20a system

Peptide Synthesis and Characterization

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Unless stated otherwise, all chemicals were purchased from commercial sources and all reactions were carried out under ambient atmosphere. Breipohl resin was obtained from Bachem (Bubendorf, Switzerland). Fmoc-amino acids were purchased from Novabiochem (EMD Chemicals, Gibbstown, USA.), Fmoc-6-Ahx-OH was acquired from Iris Biotech GMBH (Marktredwitz, Germany) and carboxyfluorescein was obtained from Acros Organics (Geel, Belgium). MilliQ was doubly deionized using a Labconco Water Pro PS purification system (18.1 MΩ).
Analytical HPLC was performed on a Shimadzu LC-20A system, equipped with a C18 ReproSil column (15 cm × 3 mm, 3 μm). A gradient program was used, 5–100% of phase B in phase A during 40 min (phase A: 100% H2O + 0.1% TFA, phase B: 100% MeCN + 0.1% TFA), flow 0.4 ml/min.
Preparative HPLC was performed on a Shimadzu LC-20A system, equipped with an NX-C18 column (15 cm × 21.2 mm, 10 µm). A gradient program was used 5–100% of phase B in phase A (phase A: 100% H2O + 0.1% TFA, phase B: 100% MeCN + 0.1% TFA), flow 6.0 ml/min.
Mass spectra were acquired on a Thermo Finnigan LCQ Advantage Max (ESI-Q) system or on a Bruker Microflex LRF (MALDI-ToF) system (with α-cyano-4-hydroxycinnamic acid matrix).

Tooyserkani R., Lipiński W., Willemsen B, & Löwik D.W. (2020). Activation of cell-penetrating peptide fragments by disulfide formation. Amino Acids, 52(8), 1161-1168.

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Quantification of Ginseng Compounds

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The fermentation broth was centrifugated and properly diluted. The concentrations of glucose and ethanol were detected using a biosensing analyzer (SBA-40C, Shandong Academy of Sciences, China). The quantification of DM-II and PPD were conducted using a SHIMADZU LC20A system (Shimadzu, Kyoto, Japan) equipped with LC-20ADXR liquid chromatograph and SIL-20AXR auto-sampler. Chromatographic separation of PPD was carried out at 30 °C on a Poroshell 120 EC-C18 column (4.6 × 250 mm, 4 μm, Agilent). DM-II and PPD were separated by using 10% water and 90% acetonitrile. The injection volume was 10 μL, and the flow rate was kept at 1.0 mL/min.

Zhu Y., Li J., Peng L., Meng L., Diao M., Jiang S., Li J, & Xie N. (2022). High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae. Microbial Cell Factories, 21, 230.

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NMR and HPLC Analysis of Natural Products

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The 1D and 2D NMR spectra were recorded on a 600 MHz Varian NMR spectrometer (VNS-600, Palo Alto, CA, USA), operated using Bruker TopSpin software (Billerica, MA, USA), at the Core Research Support Center for Natural Products and Medical Materials (CRCNM). The acquired data were processed using MestReNova 12.0.3 software (Mestrelab Research SL, Santiago de Compostela, Spain). Analytical HPLC was conducted using a Shimadzu LC-20A system (Kyoto, Japan) equipped with a Shimadzu SPD-M20A photodiode array (PDA) detector (Kyoto, Japan) and an Alltech 3300 evaporative light scattering detector (ELSD, Essen, Germany), with a Phenomenex Luna 5 μm C₁₈ column (100 Å, 250 × 4.6 mm, 1 mL/min). Deuterated solvents, including 3-(trimethylsilyl)-1-propanesulfonic acid solution (DSS, an internal calibrant, 1 wt% in D₂O, 99.9 atom % D) and sodium deuteroxide solution (NaOD, 40 wt% in D₂O, 99.5 atom % D) for NMR experiments, as well as monopotassium phosphate (KH₂PO₄, ≥99.0%), were purchased from Sigma-Aldrich. Azelaic acid (98%), sebacic acid (98%), and glycerophosphocholine (choline alfoscerate, 98%) were obtained from AK Scientific. Choline sulfate (98%) and phosphocholine chloride calcium salt tetrahydrate (>98%) were purchased from Cambridge Isotope Laboratories and TCI Chemicals, respectively. All solvents were of ACS grade or better.

Kil Y.S., Han A.R., Hong M.J., Kim J.B., Park P.H., Choi H, & Nam J.W. (2022). 1H NMR-Based Chemometrics to Gain Insights Into the Bran of Radiation-Induced Colored Wheat Mutant. Frontiers in Nutrition, 8, 806744.

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HPLC Analysis of Phenolic Compounds

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The phenolic profile was produced according to the method described previously by Pu^{24 (link)}, with some modification. HPLC analysis was performed on a Shimadzu (Kyoto, Japan) LC-20A system equipped with two pumps (LC-20AB), an injector (SIL-20A), and a detector (SPD-M20A). Separation was performed on a ZORBAX SB-C18 column (I.D. 250 × 4.6 mm, Agilent, USA). The column oven temperature was set to 30 °C. The absorbance was monitored at 253, 279, 284, 310, 324, 327, 355, and 370 nm. The solvent system had a constant flow rate of 0.7 mL/min and a 10 μL injection volume. Solvents such as 0.5% formic acid (eluant B) and methanol (eluant A) were used for the mobile phase. The gradient programs for the samples were as follows: 0–6 min, 90% B; 6–10 min, 90–80% B; 10–11 min, 80–75% B; 11–15 min, 75–70% B; 15–25 min, 70–60% B; 25–32 min, 60–45% B; 32–40 min, 45–0% B; 40–50 min, 0% B; 50–51 min, 0–90% B; 51–60 min, 90% B. The injection volume was maintained at 10 μL, and the constant temperature remained at approximately 30 °C. Detection wavelengths were chosen based on the absorption maximums of the UV spectra of the selected phenolic compounds.

Yan M., Wang Y., Watharkar R.B., Pu Y., Wu C., Lin M., Lu D., Liu M., Bao J, & Xia Y. (2022). Physicochemical and antioxidant activity of fruit harvested from eight jujube (Ziziphus jujuba Mill.) cultivars at different development stages. Scientific Reports, 12, 2272.

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Extraction and HPLC Analysis of Alginate from WT and algU Mutant Strains

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Psg WT, the algU mutant, and the algU-complemented strain were cultured in HSC for 7 days. Culture supernatant was obtained by centrifugation (12,000 × g for 5 min). Cell pellets were dried at 65 °C and weighed. The 500 µl of supernatants were extracted twice with 500 µl of ethyl acetate and 25 µl of HCl, and the organic phase was transferred to a new microcentrifuge tube. The sample was dried by centrifugal evaporator at 55 °C, and the dried sample was dissolved with 0.05% trifluoroacetic acid (TFA)/acetonitrile (9:1, v/v). The culture supernatant was analyzed by HPLC with a Shimadzu LC20A system equipped with a Symmetry C8 column (4.6 × 250 mm; Waters Corporation, MA, USA) as described previously (Sakata et al., 2021 (link)).

Nguyen V.T., Sakata N., Usuki G., Ishiga T., Hashimoto Y, & Ishiga Y. (2021). Multiple virulence factors regulated by AlgU contribute to the pathogenicity of Pseudomonas savastanoi pv. glycinea in soybean. PeerJ, 9, e12405.

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Quantifying TAL and Phloroglucinol in Supernatant

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The concentration of TAL and phloroglucinol in the cell-free supernatant were measured by HPLC using a Shimadzu LC-20A system equipped with a photodiode array detector (Shimadzu Corp., Kyoto, Japan) operating at 280 nm (for TAL) or 265 nm (for phluroglucinol). Separation was achieved using a Waters Symmetry C18 column (250 × 4.6 mm, 5 µm) (Waters, USA) working at 30 °C with the mobile phase of 20% acetonitrile (containing 0.1% acetic acid) at a flow rate of 0.6 mL/min (for TAL) and the mobile phase of 10% methanol at a flow rate of 0.6 mL/min (for phloroglucinol).

Li H., Chen W., Jin R., Jin J.M, & Tang S.Y. (2017). Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products. Microbial Cell Factories, 16, 187.

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Purification and Antioxidant Evaluation

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After separation by GFC, the fractions were further purified using reverse-phase high performance liquid chromatography (RP-HPLC). The lyophilized peptides were dissolved in distilled water at a concentration of 20 mg/mL and then injected into a Shimadzu LC-20A system (Shimadzu Corporation, Tokyo, Japan). The samples were eluted using 0.03% trifluoroacetic acid (TFA) in water (A) and 100% acetonitrile containing 0.03% TFA (B) at a flow rate of 0.8 mL/min. The following elution gradient was used: 0–10 min, linear gradient 0%–10% B; 10–20 min, linear gradient 10%–35% B; 20–55 min, linear gradient 35%–70% B. The fractions were detected at 280 nm, collected, freeze-dried and subjected to antioxidant assays.

Zhang N., Zhang C., Chen Y, & Zheng B. (2016). Purification and Characterization of Antioxidant Peptides of Pseudosciaena crocea Protein Hydrolysates. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(1), 57.

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Screening and Characterization of Ferulic Acid-Hydrolyzing Mutants

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Mutants and original clones were transferred to screening plates that contained 0.15% (m/v) ethyl ferulate and 50 μg/mL Kana and incubated at 37 °C for ten hours. The activity of clones was identified preliminarily by the larger size of the halos around the clones. The positive mutants were rescreened by detecting the enzymatic hydrolysate ferulic acid (FA) using high-performance liquid chromatography (HPLC) (LC-20A system, Shimadzu, Kyoto, Japan). The purified enzyme was added to 100 Mm Tris-HCl buffer (pH 8.6) with 1 mM MFA at 60 °C for 3 min, and the reaction was terminated by boiling for 5 min. HPLC was carried out on a Zorbax SB-C18 column at 30 °C, employing water containing 1% acetic acid as solvent A and methanol as solvent B at a rate of 0.5 mL/min. The identified positive mutants were confirmed by sequencing.

Li L., Ding L., Shao Y., Sun S., Wang M., Xiang J., Zhou J., Wu G., Song Z, & Xin Z. (2023). Enhancing the Hydrolysis and Acyl Transfer Activity of Carboxylesterase DLFae4 by a Combinational Mutagenesis and In-Silico Method. Foods, 12(6), 1169.

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EGCG Separation by HPLC

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Chromatographic separation was achieved on an Inertsil ODS column (4.6×250 mm, 5 µm; GL Sciences, Japan) using a Shimadzu LC–20 A system (Kyoto, Japan) at ambient temperature. Samples were filtered over 0.22 µm filters (Millipore) prior to injection. The mobile phase consisted of 0.1% formic acid in 40% methanol (HPLC grade) in water. The injection volume was 10 μL and the flow rate was maintained at 0.5 mL/min in an isocratic elution mode. EGCG and its oxidized species were detected at 280 nm.

An T.T., Feng S, & Zeng C.M. (2016). Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form. Redox Biology, 11, 315-321.

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Targeted Polysulfide Metabolomics by LC-MS/MS

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LC–ESI–MS/MS analyses were performed using a Xevo TQD Triple Quadrupole Mass Spectrometer (Waters Co., Milford, MA) coupled to a Shimadzu LC-20A system (Shimadzu Co.) equipped with an autosampler (SIL-20A), a communications bus module (CBM-20A), an online degasser (DGU-20A5), two liquid chromatographs (LC-20AD), and a column oven (CTO-20A). The samples were evaluated using the Shimadzu LC-20A system on a Mightysil-C18 column (50 mm × 2.0 mm inner diameter; KANTO CHEMICAL Co.) and then eluted using methanol as the mobile phase with a linear gradient (for detection of NEM-S-NEM, 1% B at 0–1 min, 99% B at 4–5 min, 1% B at 5.1–8 min; for untargeted polysulfide omics analysis, 1% B at 0 min, 99% B at 10–12 min, 1% B at 13–18 min; for quantitative targeted polysulfide metabolomics and detection of SFN derivatives and oxidized glutathione polysulfides [GS_nG], 1% B at 0–1 min, 99% B at 7–10 min, 1% B at 10.1–15 min) in the presence of 0.1% FA at a flow rate of 0.6 mL/min at 40 °C. The mass spectrometer was operated in the positive mode with the capillary voltage and desolvation gas (nitrogen) set to 1000 V and 1000 L/h, respectively, at 500 °C. The respective adducts were detected in the multiple reaction monitoring mode using the parameters shown in Table S1.

Kasamatsu S., Owaki T., Komae S., Kinno A., Ida T., Akaike T, & Ihara H. (2023). Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts. Redox Biology, 67, 102875.

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